An improved zinc-finger nuclease architecture for highly specific genome editing

By Jeffrey C. Miller, M. Holmes, Jianbin Wang, Dmitry Y. Guschin, Ya-Li Lee, I. Rupniewski, C. Beauséjour, A. Waite, Nathaniel S. Wang, Kenneth A Kim and 3 more. Nature Biotechnology, 2007

1,075 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/107962c53f8d92f2502c512a93adc03146abd12d

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Citations in the corpus

Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification (2008, neutral): “To test whether OPEN ZFNs induce mutations by NHEJ at endogenous human genes, we employed a CEL I nuclease-based mutation detection assay (Figure 3A) (Lombardo et al., 2007; Miller et al., 2007 We also tested whether OPEN ZFNs induced mutations in endogenous plant genes.”
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing (2014, background): “For example, the residual mutagenic activity observed in the presence of single gRNAs might be further reduced or eliminated by constructing RFNs that harbor obligate heterodimeric FokI domains 27–29 and other orthologous Cas9/gRNA pairings 30 .”
Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases (2009, background): “; complete sequences of the ZFNs, which carried obligate heterodimer forms of the FokI endonucleas”
Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations (2011, neutral): “…even more significant in the context of unbiased genome-wide analysis in order to distinguish between genetic background noise and disease relevant effects, considering that the expression of 5% of genes is thought to be linked to genetic variation (Montgomery et al., 2010; Pickrell et al., 2010).”
Revealing Off-Target Cleavage Specificities of Zinc Finger Nucleases by In Vitro Selection (2011, methodology): “The 3×Flag-tagged zinc-finger proteins for CCR5-224 and VF2468 were expressed as fusions to FokI obligate heterodimer”
Genome Engineering With Zinc-Finger Nucleases (2011, background): “The effect has been greatly ameliorated by the introduction of substitutions in the dimer interface of the cleavage domain that prevent homodimerization, but allow heterodimers to form (Miller et al. 2007; Szczepek et al. 2007; Sollu et al. 2010).”
Genome editing with engineered zinc finger nucleases (2010, background): “Moreover, the dependence on productive dimerization has spurred the development of variants that cleave only as a heterodimer pair, thus improving specificity via the elimination of unwanted homodimer”
Development and Applications of CRISPR-Cas9 for Genome Engineering (2014, neutral): “, 2006), zinc finger (ZF) nucleases based on eukaryotic transcription factors (Urnov et al., 2005; Miller et al., 2007), transcription activator-like effectors (TALEs) from Xanthomonas bacteria (Christian et al.”