Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

By D. Hockemeyer, F. Soldner, C. Beard, Qing Gao, M. Mitalipova, Russell Dekelver, George E. Katibah, Ranier Amora, E. A. Boydston, B. Zeitler and 7 more. Nature Biotechnology, 2009

1,106 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/24d2d2f6fd2cc5b2a7adabbefde2f1cf58e9da7a

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In Molecular Biology

Citations in the corpus

Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations (2011, positive): “Analysis of off-target cleavage by ZFNs, which results in NHEJ-mediated indels, was performed essentially as described before (Doyon et al., 2008; Hockemeyer et al., 2009; Perez et al., 2008) and is described in detail in the Extended Experimental Procedures.”
RNA-Guided Human Genome Engineering via Cas9 (2013, methodology): “We expect that RNA-guided genome targeting will have broad implications for synthetic biology (22, 23), the direct and multiplexed perturbation of gene networks (13, 24), and targeted ex vivo (25-27) and in vivo gene therapy (28).”
Somatic cell reprogramming and transitions between pluripotent states: facts, hypotheses, unresolved issues (2010, positive): “Recently, novel gene targeting strategies using zinc-finger nucleases have overcome some of these limitations (Hockemeyer et al., 2009; Zou et al., 2009).”