Mapping of picoeucaryotes in marine ecosystems with quantitative PCR of the 18S rRNA gene.

By Fei Zhu, Ramon Massana, Fabrice Not, Dominique Marie, Daniel Vaulot. FEMS Microbiology Ecology, 2005

620 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/0e90efd70c9bfd283e5d492a7322941dcc62175e

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Citations in the corpus

Screening and evaluation of some green algal strains (Chlorophyceae) isolated from freshwater and soda lakes for biofuel production. (2015, methodology): “PCR was performed with primer pairs Euk328f-Chlo02R [55,56] as described by Somogyi et al. [57].”
Arctic microbial community dynamics influenced by elevated CO2 levels (2012, background): “Members of Prasinophyceae have been found to be the most abundant picophytoplankton (Zhu et al., 2005), although recently Cuvelier et al.”
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. (2009, methodology): “Quantification of eukaryotes within environmental samples by Q-PCR can be carried out by targeting the 18S rRNA gene (Lueders et al., 2004; Zhu et al., 2005) or the internal transcribed spacer (ITS) region (Landeweert et al.”
Protistan Grazing on Marine Bacterioplankton (2008, background): “However, in addition to activity, it is obvious that the copy number of the rDNA operon, which can vary by orders of magnitude among groups (Zhu et al. 2005), might affect the differences between rRNA and rDNA libraries.”
Analysis of rRNA gene content in the Mediterranean dinoflagellate Alexandrium catenella and Alexandrium taylori: implications for the quantitative real-time PCR-based monitoring methods (2010, methodology): “Absolute quantification methods by quantitative real-time PCR can use either DNA from a known amount of cultured cells (Saito et al. 2002; Popels et al. 2003; Hosoi-Tanabe and Sako, 2005) or plasmids containing the cloned target sequence (Fontaine and Guillot, 2002; Galluzzi et al. 2004; Zhu et al.”
Protistan assemblages across the Indian Ocean, with a specific emphasis on the picoeukaryotes (2008, background): “Part of the explanation could come from the significant variation in the copy number of the 18S rRNA gene between different taxa (Zhu et al., 2005), such that ARTICLE IN PRESS”
Phylogeny and Molecular Evolution of the Green Algae (2012, background): “The prasinophytes form a paraphyletic assemblage of at least nine clades, with the Mamiellophyceae (prasinophyte clade II) being common in mesotrophic coastal areas (Zhu et al., 2005; Countway and Caron, 2006; Marie et al., 2006) and clades VIII and IX (known exclusively from environmental sequencin”
Eukaryotic plankton diversity in the sunlit ocean (2015, background): “Note that rDNAgene copy number varies from one to thousands in single eukaryotic genomes (72, 73), precluding direct translation of rDNA read number into abundance of individual organisms.”
Quantitative PCR to estimate copepod feeding (2008, neutral): “algal species in marine planktonic and sediment environments and for investigations of protist parasites and pathogens of marine metazoans (Zhu et al. 2005; Dyhrman et al. 2006; Frischer et al. 2006; Lyons et al. 2006; Moorthi et al. 2006).”
Estimation of 18S Gene Copy Number in Marine Eukaryotic Plankton Using a Next-Generation Sequencing Approach (2019, methodology): “Zhu et al. (2005) have developed a quantitative PCR-based approach to estimate 18S gene copy number for picoeukaryotes by normalizing total copies of 18S gene in the sample with cell abundance, but the results are highly dependent on DNA extraction efficiency, primer specificity and cell…”