Systematic functional analysis of the Caenorhabditis elegans genome using RNAi

By R. Kamath, Andrew G. Fraser, Yan Dong, G. Poulin, Richard Durbin, M. Gotta, Alexander Kanapin, N. Bot, S. Moreno, M. Sohrmann and 3 more. Nature, 2003

3,743 citations recorded by the source corpus.

Subfields: Aging, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/0832311adf94b5cb01e30521dd9cfe6c2c11ef1f

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Citations in the corpus

p38 MAPK Regulates Expression of Immune Response Genes and Contributes to Longevity in C. elegans (2006, methodology): “RNAi clones were obtained from the Ahringer [56] and Vidal [57] laboratories, and sequences of the RNAi clones were confirmed by sequencing.”
Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes (2003, neutral): “5 Interleukin enhancer factor 2 Channels/transporters (4) H12C20.”
Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery. (2012, neutral): “Specifically, synchronized L1worms carrying the array were exposed to RNAi for 4 days (Kamath et al., 2003).”
Molecular Approaches Toward Resistance to Plant-Parasitic Nematodes (2008, methodology): “…in C. elegans (Fire et al. 1998) . dsRNA microinjection, soaking, and feeding protocols have been used to knock down expression of all C. elegans genes and phenotypic effects have been observed for several thousand (Fraser et al. 2000 ; Gonczy et al. 2000 ; Kamath et al. 2003 ; Maeda et al. 2001) .”
The Genome Sequence of Caenorhabditis briggsae: A Platform for Comparative Genomics (2003, background): “…lethal phenotype in systematic RNA inhibition (RNAi) screens of the C. elegans ortholog partner (Maeda et al. 2001; Piano and Gunsalus 2002; Kamath et al. 2003) show a markedly lower KA/KS ratio than do pairs for which a wild-type phenotype was observed (KA/KS, 0.0445 6 0.0340 versus…”
Integrating 'omic' information: a bridge between genomics and systems biology. (2003, background): “It was reported that genes that exhibit nonviable RNAi phenotypes (lethality or sterility) are enriched in two mountains in the transcriptome topomap [ 51 ].”
Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. (2004, methodology): “2), as opposed to the L4 stage in previous screens (Kamath et al. 2003; Simmer et al. 2003).”