Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library.

By Jean‐François Rual, J. Cerón, J. Koreth, Tong Hao, Anne-Sophie Nicot, T. Hirozane-Kishikawa, J. Vandenhaute, Stuart H. Orkin, David E. Hill, S. van den Heuvel and 1 more. Genome Research, 2004

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The recently completed Caenorhabditis elegans genome sequence allows application of high-throughput (HT) approaches for phenotypic analyses using RNA interference (RNAi). As large phenotypic data sets become available, "phenoclustering" strategies can be used to begin understanding the complex molecular networks involved in development and other biological processes. The current HT-RNAi resources represent a great asset for phenotypic profiling but are limited by lack of flexibility. For instance, existing resources do not take advantage of the latest improvements in RNAi technology, such as inducible hairpin RNAi. Here we show that a C. elegans ORFeome resource, generated with the Gateway cloning system, can be used as a starting point to generate alternative HT-RNAi resources with enhanced flexibility. The versatility inherent to the Gateway system suggests that additional HT-RNAi libraries can now be readily generated to perform gene knockdowns under various conditions, increasing the possibilities for phenome mapping in C. elegans.

Subfields: Aging, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/cd58db82d6849cfe640cf6acc00d42827d7cbeaf

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Citations in the corpus

The Concept of Synthetic Lethality in the Context of Anticancer Therapy (2005, methodology): “RNAi is a powerful method for silencing genes in worms and fruitflies, and collections of interfering RNAs have been created to facilitate high-throughput genome-wide screens in these organism”
A Role for Autophagy in the Extension of Lifespan by Dietary Restriction in C. elegans (2008, methodology): “MV, RNAi clone from Marc Vidal’s RNAi library [61]; JA, RNAi clone from Julie Ahringer’s RNAi library [60]; F, FUDR (100 lM) was added to the plates to prevent progeny production.”
p38 MAPK Regulates Expression of Immune Response Genes and Contributes to Longevity in C. elegans (2006, methodology): “RNAi clones were obtained from the Ahringer [56] and Vidal [57] laboratories, and sequences of the RNAi clones were confirmed by sequencing.”
Lifespan extension by conditions that inhibit translation in Caenorhabditis elegans (2007, background): “, 2003) except for the rsks-1 RNAi clone, which was from Marc Vidal’s RNAi library (Rual et al., 2004).”