PSD-95 involvement in maturation of excitatory synapses.

By A. El-Husseini, Eric Schnell, D. Chetkovich, R. Nicoll, D. Bredt. Science, 2000

1,311 citations recorded by the source corpus.

PSD-95 is a neuronal PDZ protein that associates with receptors and cytoskeletal elements at synapses, but whose function is uncertain. We found that overexpression of PSD-95 in hippocampal neurons can drive maturation of glutamatergic synapses. PSD-95 expression enhanced postsynaptic clustering and activity of glutamate receptors. Postsynaptic expression of PSD-95 also enhanced maturation of the presynaptic terminal. These effects required synaptic clustering of PSD-95 but did not rely on its guanylate kinase domain. PSD-95 expression also increased the number and size of dendritic spines. These results demonstrate that PSD-95 can orchestrate synaptic development and are suggestive of roles for PSD-95 in synapse stabilization and plasticity.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/02bdabbfba4306ffdaa65d599495d8d249558dbb

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In Cellular and Molecular Neuroscience

1. Karel Svoboda
2. Richard L. Huganir
3. J. Shepherd
4. M. Sheng
5. Noah W. Gray
6. Robby M. Weimer
7. I. Bureau
8. H. Hering

In Molecular Biology

1. J. Shepherd
2. Jack D. Keene
3. Richard L. Huganir
4. Noah W. Gray
5. Robby M. Weimer
6. I. Bureau
7. M. Sheng
8. Karel Svoboda

Citations in the corpus

• Ubiquitination regulates PSD-95 degradation and AMPA receptor surface expression. (2003, background): “Overexpression of PSD-95 enhances AMPA receptor- mediated synaptic transmission (Beique and Andrade, Antibodies 2003; El-Husseini et al., 2000; Schnell et al., 2002) and Antibodies used in immunoprecipitation experiments included increases the number and size of dendritic spines (El- Mdm2 (Santa…”
• Structure and function of dendritic spines. (2002, background): “Consistent with this simple picture are observations that some manipulations that modulate spine size also appear to change measures of synaptic strength, including the amplitude of miniature synaptic currents (47).”
• Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome. (2001, background): “It is remarkable that volved in maintaining the PSD structure and neuronal67% of the transcripts that both immunoprecipitate with cell signaling (El-Husseini et al., 2000; Paschen et al.,the FMRP-mRNP complex in the mouse brain and dis1994; Satoh et al., 1997; Takeuchi et al., 1997).”
• The cell biology of synaptic plasticity: AMPA receptor trafficking. (2007, background): “Overexpression of PSD-95 enhances AMPAR-mediated synaptic currents (Beique & Andrade 2003, El-Husseini et al. 2000).”
• Normal gut microbiota modulates brain development and behavior (2011, background): “Likewise, PSD-95 is involved in the maturation of excitatory synapses (24).”
• Dentritic spines : structure, dynamics and regulation (2001, background): “Overexpression of PSD95, which binds directly to NMDA receptors, increased the number and size of spines in cultured neuron”