Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.

By Michael Hofmann, Christian Eggeling, S. Jakobs, Stefan W. Hell. Proceedings of the National Academy of Sciences of the United States of America, 2005

811 citations recorded by the source corpus.

Subfields: Biophysics, Cellular and Molecular Neuroscience, Structural Biology

View Paper: https://www.semanticscholar.org/paper/0160d3c965d4e5e789ff8bd169727766a0e8c4b9

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Citations in the corpus

Image Resolution in Optical Nanoscopy (2013, methodology): “The FRC can be corrected for this effect by estimating the spurious correlation parameter Q in equation (2).”
The universal scaling laws that determine the achievable resolution in different schemes for super-resolution imaging (2012, background): “Since the metastable lifetime is infinite in RESOLFT, it is convenient to replace it with the reverse switching rate γsw (when the switching is reversible).”
A guide to super-resolution fluorescence microscopy (2010, background): “The required nonlinearity is currently achieved by using reversible saturable optical fluorescence transitions (RESOLFT) between molecular states (Hofmann et al., 2005; Hell, 2007).”
Fluorescent probes for super-resolution imaging in living cells (2008, background): “This more general approach to super-resolution imaging is termed reversible saturable optically linear fluorescence transitions (RESOLFT”
Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. (2006, background): “The conditions for effective FPALM also Biophysical Journal 91(11) 4258–4272 reveal a need for minimal spontaneous photoconversion rates in PA-FPs, as well as for RESOLFT microscopies that use PA-FPs (15).”
Far-Field Optical Nanoscopy (2007, background): “These results also verified the prediction [38–42] about the huge potential of switching photoactivatable proteins and photochromic (photoswitchable) organic dyes for overcoming far-field microscopy diffraction barrier.”
Super resolution fluorescence microscopy (2009, methodology): “Unlike STED which features a high IS value (~107 W/cm2) and thus requiring an intense depletion laser (often > 109 W/ cm2), an optical transition that has low IS can be chosen for RESOLFT, allowing superresolution imaging at a much lower depletion laser intensity (3).”
A BIRD’S EYE VIEW: TRACKING SLOW NANOMETER-SCALE MOVEMENTS OF SINGLE MOLECULAR NANO-ASSEMBLIES (2010, positive): “…overcome this optical resolution barrier and bring the localization accuracy of a single particle down to the low nanometer range (Betzig and Chichester, 1993; Heintzmann et al., 2002; Hess et al., 2006; Hofmann et al., 2005; Huang et al., 2009; Moerner, 2007; Qu et al., 2004; Rust et al., 2006).”