Knockout rats generated by embryo microinjection of TALENs

By L. Tesson, C. Usal, S. Ménoret, Elo Leung, Brett J. Niles, S. Remy, Yolanda Santiago, Anna I Vincent, Xiangdong Meng, Lei Zhang and 3 more. Nature Biotechnology, 2011

618 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/00dd8d00f2334a13a25b7bcc5410723c6928f627

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In Molecular Biology

Citations in the corpus

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering (2013, neutral): “DSBs induced by these site-specific nucleases can then be repaired by error-prone nonhomologous end joining (NHEJ) resulting in mutant mice and rats carrying deletions or insertions at the cut site (Carbery et al., 2010; Geurts et al., 2009; Sung et al., 2013; Tesson et al., 2011).”
FLASH Assembly of TALENs Enables High-Throughput Genome Editing (2012, positive): “…Repair of TALEN-induced double-strand breaks (DSBs) by either non-homologous end-joining (NHEJ) or homology-directed repair can induce efficient alteration of endogenous genes in yeast, 16 plants, 17 nematodes, 18 zebrafish, 21, 22 rats, 20 and human somatic 6, 15, 17 and pluripotent stem cells.”
Genome Engineering of Crops with Designer Nucleases (2012, background): “, 2011a), rats (Tesson et al., 2011) and humans (Miller et al.”
TALENs: a widely applicable technology for targeted genome editing (2012, neutral): “Although ZFNs and TALENs have not been directly compared, many studies have shown that TALENs and ZFNs have comparable efficiencies when targeted to the same gene 9, 13, 18, 20 .”
Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair (2014, background): “…RGNs based on the CRISPR/Cas9 system allows specific gene disruption in many organisms not previously amenable to forward genetic analyses, for instance, in common experimental models such as the rat or the zebrafish (Huang et al. 2011; Sander et al. 2011; Tesson et al. 2011; Hwang et al. 2013b).”
Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. (2014, neutral): “Mosaicism It is worth notifying that the sequence data of both cultured embryos and founder animals showed multiple genotypes (Figures 2B, 3D, and 4C), suggesting the CRISPR/Cas9-mediated cleavage had occurred multiple times at different stages of monkey embryogenesis and resulted in mosaicism of th”