Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos.

By Yuyu Niu, Bin Shen, Yiqiang Cui, Yongchang Chen, Jianying Wang, Lei Wang, Yu Kang, Xiao-Yang Zhao, W. Si, A. Xiang and 17 more. Cell, 2014

1,061 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/05da6670e90857de1cb39db85547b4a34d96c97f

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

Citations in the corpus

Delivering CRISPR: a review of the challenges and approaches (2018, background): “, 2014), disruption of two genes in cynomolgus monkeys from a single injection into one-cellstage embryos (see Figure 5(A)) (Niu et al., 2014), correction of a cataract-causing mutation in mice (Wu et al.”
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, background): “In vertebrates, coinjection of in vitro transcribed Cas9 mRNA and sgRNA into single-cell embryos can produce multi-gene, biallelic mutant animals with high efficiency; the multiple mutations can also be efficiently transmitted to the next generation [11–16].”
The new frontier of genome engineering with CRISPR-Cas9 (2014, positive): “The technology can also facilitate the generation of mouse (120–122) and rat (123, 124) models better suited to pharmacological studies and the understanding of human diseases, as well as pigs (125) andmonkeys (126).”
Development and Applications of CRISPR-Cas9 for Genome Engineering (2014, positive): “This could be applied to develop novel transgenic animal models (Wang et al., 2013; Niu et al., 2014), to engineer isogenic ES and iPS cell disease models with specific mutations introduced or corrected, respectively, or in vivo and ex vivo gene correction (Schwank et al., 2013; Wu et al., 2013).”
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes (2015, background): “…et al., 2013; Hsu et al., 2014; Hwang et al., 2013; Ikmi et al., 2014; Irion et al., 2014; Jinek et al., 2013; Li et al., 2013a; Li et al., 2013b; Long et al., 2014; Ma et al., 2014; Mali et al., 2013c; Niu et al., 2014; Smith et al., 2014a; Wu et al., 2013; Wu et al., 2014b; Yang et al., 2013).”
A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, background): “In vertebrates such as zebrafish, mice, rats, and monkeys, coinjection of gRNA and Cas9-encoding mRNA transcribed in vitro into single-cell-stage embryos can efficiently generate animals with multiple biallelic mutations that can be transmitted to the next generation with high efficiency [18,28-32].”