An improved yeast transformation method for the generation of very large human antibody libraries.

By L. Benatuil, Jennifer Perez, J. Belk, C. Hsieh. 2010

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Naïve Human Antibody Libraries for Infectious Diseases (2018, background): “Examples of other in vitro display technologies that can be used for mAb generation are ribosomal display [54], yeast surface display [55, 56], bacterial surface display [57, 58] and mRNA display [62, 63].”
Deep learning of the regulatory grammar of yeast 5′ untranslated regions from 500,000 random sequences (2017, positive): “For the library transformation into yeast, we followed the electro-poration protocol described (Benatuil et al. 2010).”
Sampling and energy evaluation challenges in ligand binding protein design (2017, neutral): “Libraries were transformed as linear PCR product together with linear cut pETCON(digested with NdeI and XhoI) in to EBY100 yeast cells by electroporation.(29)”
Computational design of environmental sensors for the potent opioid fentanyl (2017, background): “Multipose binding has been observed in many naturally occurring protein-ligand complexes (Kulp et al., 2012; Blum et al., 2011; Barelier et al., 2015), but for our approach we sought precise control of the fentanyl pose by considering only a single conformer per protein scaffold.”
Improving de novo protein binder design with deep learning (2022, neutral): “The resulting DNA inserts and linearized pETcon3 vector were transformed into EBY100 yeast following an established protocol 37 .”