Addressing polyspecificity of antibodies selected from an in vitro yeast presentation system: a FACS-based, high-throughput selection and analytical tool.

By Yingda Xu, W. Roach, Tingwan Sun, T. Jain, B. Prinz, Ta-Yi Yu, J. Torrey, Jerry M Thomas, P. Bobrowicz, M. Vásquez and 2 more. 2013

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Biophysical properties of the clinical-stage antibody landscape (2017, methodology): “Assays performed in this study included assessment of antibody selfinteraction by AC-SINS (affinity-capture self-interaction nanoparticle spectroscopy) (3, 9, 11) and CSI-BLI (clone self-interaction by biolayer interferometry) (7), a variety of metrics of cross-interaction such as binding PSR (poly-”
Nonspecificity in a nonimmune human scFv repertoire (2017, methodology): “SMPs were prepared as described previously.(17) Briefly, one billion HEK or Sf9 cells were pelleted, washed and homogenized.”
Efficient evolution of human antibodies from general protein language models (2023, unknown): “For each of the seven antibodies, we tested the wild-type alongside three affinity-matured variants using a polyspecificity assay that assesses non-specific binding to soluble membrane proteins (Methods) 41,42 .”