Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses.

By G. Nagel, M. Brauner, J. Liewald, Nona Adeishvili, E. Bamberg, A. Gottschalk. Current Biology, 2005

975 citations recorded by the source corpus.

Subfields: Aging, Cellular and Molecular Neuroscience, Endocrine and Autonomic Systems

View Paper: https://www.semanticscholar.org/paper/fb73bb2cd8872be2e7d0527aac0a8b92be127c6c

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Citations in the corpus

Characterization of engineered channelrhodopsin variants with improved properties and kinetics. (2009, background): “We also characterized ChD and the H134R mutant of ChR2, which was previously reported to have a reduced level of inactivation ( 19 ,20).”
Multimodal fast optical interrogation of neural circuitry (2007, background): “Author Information The GenBank accession number is EF474018 for the ‘mammalianized’ NpHR sequence and EF474017 for the ‘mammalianized’ ChR2(1-315) sequence.”
The Development and Application of Optogenetics (2011, background): “…with substitution of histidine for arginine at position 134 to increase steady-state current size, although the mechanism of the latter effect (delayed channel closure) also significantly impaired temporal precision and high-speed spiking (ChR2-H134R; Nagel et al. 2005, Gradinaru et al. 2007).”
Multiple-Color Optical Activation, Silencing, and Desynchronization of Neural Activity, with Single-Spike Temporal Resolution (2007, background): “elegans have devised ways of delivering all-trans-retinal to the nervous systems of such animals in order to enable ChR2 function [54,55], and these protocols would therefore also enable the function of Halo in these invertebrates.”
Working with Worms: Caenorhabditis elegans as a Model Organism (2019, methodology): “Additionally, like other systems, channelrhodopsin requires all-trans retinal (ATR; Nagel et al., 2005) to form functional channels, so incorporate ATR into E. coli immediately prior to seeding colony plates (add 1 µl of 100 mM ATR dissolved in ethanol to 250 µl of E. coli OP50 suspension…”