Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis

By Caryn E Outten, and Thomas V. O'Halloran. Science, 2001

1,479 citations recorded by the source corpus.

Subfields: Health, Toxicology and Mutagenesis, Nutrition and Dietetics, Plant Science

View Paper: https://www.semanticscholar.org/paper/faa4d795bfe0af88aee9cc191a3e5bfd3df7f2f2

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Health, Toxicology and Mutagenesis

1. S. Clemens
2. K. Waldron
3. Taiho Kambe
4. D. Eide
5. D. Nies
6. N. Robinson
7. Ayako Hashimoto
8. J. Imlay

In Nutrition and Dietetics

1. S. Clemens
2. K. Waldron
3. Taiho Kambe
4. Jay C. D. Hinton
5. D. Eide
6. D. Nies
7. N. Robinson
8. Sacha Lucchini

In Plant Science

1. S. Clemens
2. Ute Krämer
3. K. Waldron
4. D. Eide
5. D. Nies
6. N. Robinson
7. J. Imlay
8. J. Ma

Citations in the corpus

• The MerR family of transcriptional regulators. (2003, background): “These arguments and experiments are complicated by the fact that estimated free metal ion concentrations in the cytoplasm are extremely low [108].”
• Metalloproteins and metal sensing (2009, background): “A keystone of this article will be that metal availability is controlled in the cytoplas”
• Lag Phase Is a Distinct Growth Phase That Prepares Bacteria for Exponential Growth and Involves Transient Metal Accumulation (2011, methodology): “It is now possible to audit many metals simultaneously using the highly sensitive inductively coupled plasma mass spectrometry (ICP-MS) method, which simultaneously detects 36 metals associated with bacterial cells (85).”
• THE ELEMENTAL COMPOSITION OF SOME MARINE PHYTOPLANKTON 1 (2003, neutral): “The average cellular concentrations of Fe, Cu, and Mo in phytoplankton (Table 2) are similar to those reported for Escherichia coli (Outten and O’Halloran 2001), but the concentration of Mn is almost 100 times higher and that of Zn 10 times lower.”
• Biogeochemical signatures through time as inferred from whole microbial genomes (2005, background): “Previous work indicates that E. coli exert tight genetic control over cellular metal concentrations, even for relatively low-toxicity metals such as zinc (Outten and O’Halloran, 2001).”
• Efflux-mediated heavy metal resistance in prokaryotes. (2003, background): “coli by Outten and O’Halloran [70] ; in this bacterium, the apparent concentration of Zn2þ is kept at 0.”
• Structure of a bacterial multidrug ABC transporter (2006, background): “For example, there is o1 free Zn2þ ion per cell (Outten and O’Halloran, 2001); the corresponding numbers for Ca2þ are B30, Mg2þ B90 000, and for Naþ and Kþ 42 106 (Maguire and Cowan, 2002).”
• The Physiological, Biochemical, and Molecular Roles of Zinc Transporters in Zinc Homeostasis and Metabolism. (2015, background): “Zinc is, however, bound with a myriad of proteins and sequestered into organelles and vesicles, and thus the cytosolic labile (“free”) zinc ion concentration is very low and is considered to range between the picomolar and low nanomolar (305, 337, 366, 425) (FIGURE 3).”
• Functions and Homeostasis of Zinc, Copper, and Nickel in plants (2005, background): “Based on results from bacterial metal sensors and metallochaperones, the concentrations of free, hydrated metal ions in the symplasm of plant cells are believed to be in the femtomolar and attomolar range for Zn and Cu ions, respectively (Rae et al. 1999; O'Halloran and Culotta 2000; Outten and O'Ha”