Proliferating Cells Express mRNAs with Shortened 3' Untranslated Regions and Fewer MicroRNA Target Sites

By Rickard Sandberg, J. Neilson, Arup Sarma, Phillip A. Sharp, C. Burge. Science, 2008

1,389 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/fa866aeebfe068e99c597aff223e20995a00c832

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. David P. Bartel
2. Eugene Berezikov
3. Carlo Maria Croce
4. C. Harris
5. A. Lund
6. Christine Mayr
7. Gianpiero di Leva
8. A. Robles

In Molecular Biology

1. David P. Bartel
2. Carlo Maria Croce
3. Eugene Berezikov
4. C. Harris
5. Gianpiero di Leva
6. Rickard Sandberg
7. A. Robles
8. Bríd Ryan

Citations in the corpus

• An Abundance of Ubiquitously Expressed Genes Revealed by Tissue Transcriptome Sequence Data (2009, background): “Previous studies using ESTs and microarrays have found a bias towards the usage of longer 39UTRs in brain tissues [14,37] and found that 39UTR length can be dynamically regulated in response to activating and mitogenic signals [38].”
• siRNA vs. shRNA: similarities and differences. (2009, background): “sites [128] in highly proliferating tumors with modulated feedback mechanisms is not known.”
• Ending the message: poly(A) signals then and now. (2011, background): “Several independent studies have revealed the remarkable fact that either levels of cell proliferation or developmental stage can cause a shift in APA from more distal to more proximal PAS selection (Sandberg et al. 2008; Ji et al. 2009; Mayr and Bartel 2009).”
• Sequence signatures and mRNA concentration can explain two-thirds of protein abundance variation in a human cell line (2010, background): “In comparison, short 30UTRs have on average fewer binding sites for potential repressor molecules, such as miRNAs and RNAbinding proteins, than long 30UTRs, and thus a short sequence may be advantageous for high protein abundance (Sandberg et al, 2008; Mayr and Bartel, 2009; Santhanam et al, 2009).”
• Genetic variation in microRNA networks: the implications for cancer research (2010, positive): “However, there is evidence that proliferating cells have shorter 3′ UTRs, fewer miRNA binding sites and therefore diminished regulation by miRNA”
• Widespread shortening of 3′UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells (2009, positive): “The tandem UTR length index (TLI) is a relative measure of 3 0 UTR length derived from array data (Sandberg et al., 2008 ).”
• The microRNA miR-29 controls innate and adaptive immune responses to intracellular bacterial infection by targeting interferon-γ (2011, background): “Furthermore, proliferating T cells express genes with shorter 3′ untranslated regions (UTRs) than those in resting T cells, which renders these mRNAs less susceptible to regulation by miRNAs owing to the loss of miRNA-binding site”
• Mechanisms and consequences of alternative polyadenylation (2011, neutral): “During T cell activation, for example, CR-APA events occur at both early and late stages of activation (Sandberg et al., 2008).”
• A majority of m6A residues are in the last exons, allowing the potential for 3′ UTR regulation (2015, background): “The earlier findings (Sandberg et al. 2008; Ji et al. 2009) on the choice of polyA sites and the consequent exchange in the length of 3′ UTRs that occurs with differentiation and the more proximal site usage with rapid growth led us to examine the density of m6A residues in the last exons in the…”