Cre-lox-regulated conditional RNA interference from transgenes.

By A. Ventura, Alexander Meissner, C. Dillon, Michael Mcmanus, Phillip A. Sharp, L. van Parijs, R. Jaenisch, T. Jacks. Proceedings of the National Academy of Sciences of the United States of America, 2004

671 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/fa687d18b2f8e10f97685289aecd77783a2f1782

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Citations in the corpus

In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state (2007, background): “d, Western blot analysis for Oct4 and Nanog expression of different Oct4 iPS clones (6, 9, 10, 16, 18) and a GFP-labelled subclone of clone 18 (18.”
Direct cell reprogramming is a stochastic process amenable to acceleration (2009, background): “28 Ventura, A. et al., Cre-lox-regulated conditional RNA interference from transgenes. Proc Natl Acad Sci U S A 101 (28), 10380-10385 (2004).”
Mutant p53 gain of function in two mouse models of Li-Fraumeni syndrome. (2004, background): “…to be an oncogene due to its ability to cooperate in the trans-therefore utilized a conditional lentiviral shRNA expression construct, pSico-p53 (Ventura et al., 2004), to knock formation of cells (Eliyahu et al., 1984; Parada et al., 1984) and its overexpression in tumors and transformeddown…”
CRISPR interference (CRISPRi) for sequence-specific control of gene expression (2013, methodology): “To express sgRNAs in mammalian cells, we have cloned a pSico-derived lentiviral mouse U6-based expression vector 58 (Addgene ID no. 44248).”
Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. (2008, background): “, 2007) and expressed a p53 hairpin (Ventura et al., 2004).”
Impaired microRNA processing enhances cellular transformation and tumorigenesis (2007, methodology): “To investigate this possibility, we generated a shRNA against Dicer1 flanked by loxP sites (pSico R -shDicer1), allowing subsequent removal of the hairpin via Cre-mediated recombinatio”
Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysis (2005, methodology): “Lentiviral infections of ES cells were performed as described previously (34).”
CREB/ATF-dependent T cell receptor–induced FoxP3 gene expression: a role for DNA methylation (2007, neutral): “The plasmids encoding shRNA against Dnmt1 were provided by T. Jacks (Massachussetts Insitute of Technology, Cambridge, MA) (29).”