Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti.

By T. Kaneko, Yasukazu Nakamura, Shusei Sato, E. Asamizu, Tomohiko Kato, S. Sasamoto, A. Watanabe, K. Idesawa, Atsuko Ishikawa, K. Kawashima and 14 more. DNA Research, 2000

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The complete nucleotide sequence of the genome of a symbiotic bacterium Mesorhizobium loti strain MAFF303099 was determined. The genome of M. loti consisted of a single chromosome (7,036,071 bp) and two plasmids, designated as pMLa (351,911 bp) and pMLb (208, 315 bp). The chromosome comprises 6752 potential protein-coding genes, two sets of rRNA genes and 50 tRNA genes representing 47 tRNA species. Fifty-four percent of the potential protein genes showed sequence similarity to genes of known function, 21% to hypothetical genes, and the remaining 25% had no apparent similarity to reported genes. A 611-kb DNA segment, a highly probable candidate of a symbiotic island, was identified, and 30 genes for nitrogen fixation and 24 genes for nodulation were assigned in this region. Codon usage analysis suggested that the symbiotic island as well as the plasmids originated and were transmitted from other genetic systems. The genomes of two plasmids, pMLa and pMLb, contained 320 and 209 potential protein-coding genes, respectively, for a variety of biological functions. These include genes for the ABC-transporter system, phosphate assimilation, two-component system, DNA replication and conjugation, but only one gene for nodulation was identified.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/f97df799fc61da56821c8b0ea577ef20f9e56ef6

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Citations in the corpus

Bacteria with ACC deaminase can promote plant growth and help to feed the world. (2014, background): “…Mesorhizobium loti have been found to be under the transcriptional control of the nifA promoter that is normally responsible for activating the transcription of nif, nitrogen fixation genes (Kaneko et al. 2000; Nascimento et al. 2012a; Nukui et al. 2006; Sullivan et al. 2002; Uchiumi et al. 2004).”
Score-based prediction of genomic islands in prokaryotic genomes using hidden Markov models (2006, background): “These patches have been called genomic islands (GI) or due to their role and more specifically pathogenicity islands [2] or symbiotic islands [3].”
Mechanism of Phosphate Solubilization and Physiological Functions of Phosphate-Solubilizing Microorganisms (2014, background): “…strains of M. loti have been found to be under the transcriptional control of the nifA promoter that is normally responsible for activating the transcription of nif, nitrogen fixation genes (Kaneko et al. 2000; Sullivan et al. 2002; Uchiumi et al. 2004; Nukui et al. 2006; Nascimento et al. 2012).”
ω-Amino Acid:Pyruvate Transaminase from Alcaligenes denitrificans Y2k-2: a New Catalyst for Kinetic Resolution of β-Amino Acids and Amines (2004, background): “loti, mll1632 (9), was cloned and overexpressed in E.”