Physiological heterogeneity in biofilms

By P. Stewart, M. Franklin. Nature Reviews Microbiology, 2008

2,313 citations recorded by the source corpus.

Subfields: Ecology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f754b7dd9519533e5e469f3b1705ce97f9f63fc2

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

In Genetics

In Molecular Biology

Citations in the corpus

Probiotics and competitive exclusion of pathogens in shrimp aquaculture (2020, background): “Nevertheless, several vibriocins (bacteriocins produced by the genus Vibrio; Wahba, 1965; McCall & Sizemore, 1979; Sugita et al., 1997; Shehane & Sizemore, 2002) and bacteriocin-like inhibitory substances (Prasad et al., 2005; Carraturo et al., 2006) involved in inter-strain and inter-species…”
Assembly and Development of the Pseudomonas aeruginosa Biofilm Matrix (2009, background): “Perhaps nutrient gradients in the microcolony [34,35] lead to gradients of intracellular cyclic-diguanylate from the periphery (highest) to the center of the microcolony (lowest), which may explain the changes in Psl localization.”
Microbial Surface Colonization and Biofilm Development in Marine Environments (2015, background): “The microenvironment near a submerged surface is highly heterogeneous in that multiple gradients exist, including gradients of oxygen, pH, osmolarity, electron donors, electron acceptors, metabolizable substrates, redox potential, and chemical cues, including chemotactic attractants or repellents (3”
A field comparison of multiple techniques to quantify groundwater–surface-water interactions (2015, background): “…from laboratory experiments or other sites may lead to systematic bias because these reactions are highly variable, and the decisive factors controlling the kinetics (including microorganisms) are not known decisively (Stewart and Franklin 2008, Flemming and Wingender 2010, Besemer et al. 2012).”
Biofilms as Promoters of Bacterial Antibiotic Resistance and Tolerance (2020, background): “Oxygen and nutrient depletion occur in particular niches, such as inside the microcolonies and in the lower cell layers, and these conditions can induce a variety of physiological states involving different metabolism (aerobic, microaerobic, and fermentative) and growth rates (fast and slow growth, ”
Microswimming in viscoelastic fluids (2021, background): “While many studies model complex fluids as continuum media, it is important to realize that most biological examples are highly heterogeneous at the microscopic scale [68].”
A review of microbial biofilms of produce: Future challenge to food safety (2012, background): “Bacteria express genetic heterogeneity such as microscale chemical gradients, adaptation to local environmental conditions, stochastic gene expression and mutations, gene transfer, and other genetic variations (151).”
Biofilm-Related Infections: Bridging the Gap between Clinical Management and Fundamental Aspects of Recalcitrance toward Antibiotics (2014, background): “Dispersin B is a hexosaminidase produced by Aggregatibacter actinomycetemcomitans that hydrolyzes PNAG, an important component of S. epidermidis ECM.”
The importance of the viable but non-culturable state in human bacterial pathogens (2014, background): “The microenvironment inside the biofilm structure is subjected to oxygen, pH and nutritional stresses; there is a lower nutrient availability and higher waste concentration deeper inside the biofilm than in the periphery (Stewart and Franklin, 2008).”
Extracellular DNA Chelates Cations and Induces Antibiotic Resistance in Pseudomonas aeruginosa Biofilms (2008, result): “Our observation that DNA imposes a cation gradient in biofilm is also consistent with previous reports of oxygen and nutrient gradients within biofilms, which result in diverse physiological cellular states within a biofilm community [72].”
Targeting bacterial membrane function: an underexploited mechanism for treating persistent infections (2010, background): “tuberculosis to treat chronic TB; similarly, as membrane-mediated anaerobic metabolism is increasingly being recognized as essential for the maintenance of biofilm”