Improving quantitative real-time RT-PCR reproducibility by boosting primer-linked amplification efficiency

By Ales Tichopád, A. Džidić, M. W. Pfaffl. Biotechnology Letters, 2002

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Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s CT difference” formula (2006, methodology): “Besides these technical aspects, novel algorithms (such as the sigmoidal and the logistic models [21, 25, 27]) have been developed in recent years to include more data points in the data analysis, increasing its precision.”
Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction (2005, methodology): “Currently, there are several different methods for estimating CT: the fit point, Taqman threshold, SDM (Tichopad et al., 2002, 2003, 2004; Wittwer et al., 2001), and FDM (Tichopad et al., 2004) methods.”
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data (2009, background): “y0 or Fb) that represents a constant (6, 27 ,28) or cycle-dependent baseline (5,19).”
RNA integrity and the effect on the real-time qRT-PCR performance. (2006, background): “And because of exponential amplification of the initial information, any extant error is amplified, too (Tichopad et al., 2002).”