Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression.
By Lei S. Qi, Matthew H. Larson, Luke A. Gilbert, Jennifer A. Doudna, Jonathan S. Weissman, Adam P. Arkin, Wendell A. Lim. Cell, 2013
4,744 citations recorded by the source corpus.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/c5fb3cb9b177bdcb3b44175e89b4e18c8cedf82d
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In Molecular Biology
- Feng Zhang
- Jennifer A. Doudna
- Lei S. Qi
- P. Hsu
- J. Keith Joung
- Haifeng Wang
- Marie La Russa
- Rodolphe Barrangou
Citations in the corpus
- CRISPR RNA-guided activation of endogenous human genes (2013, background): “RNA-mediated recruitment of a catalytically inactive mutant form of Cas9 (referred to as dCas9) can induce repression of endogenous genes in bacterial and human cells 8,9 .”
- Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems (2016, methodology): “The dCas9 has been used for transcriptional activation and repression [134-137], for localizing fluorescent protein labels [138], and for recruiting histone modifying enzymes [139, 140].”
- Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice (2013, neutral): “in bacteria (30,32), yeast (33), zebrafish (34–36), fruit fly (37) and human cells (28,29,38)].”
- A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response (2016, methodology): “coli MG1655-derived strain by lambda Red recombinase-mediated recombineering as described previously (Qi et al., 2013), and moved from the MG1655-derived strain into the dCas9expressing BW25113 strain by P1 transduction and selection on kanamycin following established protocols.”
- CRISPR technology: A decade of genome editing is only the beginning (2023, background): “As a result, the first examples of engineered CRISPR-Cas involved transcriptional repression or activation to silence or up-regulate specific genes (8, 9).”
- CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes (2013, background): “dCas9 Fusion Proteins Can Efficiently Activate or Silence Transcription We have shown recently that CRISPRi can decrease gene expression in human cells (Qi et al., 2013).”