A monomeric red fluorescent protein

By Robert E. Campbell, Oded Tour, A. Palmer, Paul A. Steinbach, G. Baird, D. Zacharias, Roger Tsien. Proceedings of the National Academy of Sciences of the United States of America, 2002

2,460 citations recorded by the source corpus.

Subfields: Biophysics, Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f2fcb1844e9baf5688bd419aa815e1b0ca9b53ee

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Citations in the corpus

Photoactivatable fluorescent proteins (2005, background): “10 ), which are red photoactivatable variants of a monomeric versio”
Global analysis of protein localization in budding yeast (2003, background): “Haploid reference strains expressing monomeric red fluorescent protein (mRFP”
The growing and glowing toolbox of fluorescent and photoactive proteins (2016, background): “Given that most FPs are naturally dimeric or tetrameric, substantial effort has also been aimed at engineering monomeric variants [5].”
Mitochondrial β‐oxidation in Aspergillus nidulans (2004, methodology): “The gene encoding a monomeric variant of the Discosomal rfp was PCR amplified from mRFP1 (Campbell et al., 2002) and cloned into pAN52-3 at the HindIII site.”
Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses. (2006, neutral): “The cDNA for mRFP1 was amplified by PCR from mRFP1(Campbell et al., 2002) in pHRSET-B using oligos 5 & 6 and inserted into pEGFP-C1 using Age I and Not I, or into pEGFP-N1 at Age I and Bgl II by using oligos 7 & 8.”
Cloning Vectors for Rice (2009, background): “pUbi maize Ubiquitin-1promoter containing the promoter, first exon, and first intron; Tnos nos terminator; sGFP synthetic green fluorescent protein; eGFP enhanced green fluorescent protein (Clontech, USA); mRFP monomeric red fluorescent protein [5]; HA HA tag; Myc Myc tag”
A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis (2011, methodology): “Each pair of PCR primers was engineered with the appropriate 39 and 59 restriction sites for cloning and sequence for either a 9-, 12-, or 13amino-acid linker between the target protein and FP, as described previously [65].”
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. (2013, methodology): “We constructed a red fluorescent protein (mRFP)-based reporter system (Campbell et al., 2002) and inserted it into the E. coli MG1655 genome.”
Nucleo-cytoplasmic distribution of β-catenin is regulated by retention (2006, methodology): “For red fluorescent fusion proteins, cDNAs were inserted into mRF-N2 or mRF-C2, in which the parental mYFP was replaced by aa 1-225 of monomeric red fluorescent protein 1 (mRFP1) (Campbell et al., 2002).”
Vacuolar H+-ATPase Activity Is Required for Endocytic and Secretory Trafficking in Arabidopsis[W] (2006, methodology): “The presence of VHA-a1–GFP in BFA compartments suggests that it is localized either in endosomes or in a trans-Golgi compartment; therefore, we performed colocalization experiments with appropriate marker proteins fused to monomeric red fluorescent protein (mRFP) (Campbell et al., 2002).”
Design, construction and characterization of a set of insulated bacterial promoters (2010, background): “The second was dsRed, which is an engineered fluorescent protein with little homology to GFP (42).”
Inclusion body formation reduces levels of mutant huntingtin and the risk of neuronal death (2004, methodology): “Along with Htt ex1 -GFP, neurons were co-transfected with a monomeric red fluorescent protein (mRFP”