In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro

By Chun-Chi Liang, Ann Y. Park, J. Guan. Nature Protocols, 2007

4,613 citations recorded by the source corpus.

Subfields: Cell Biology, Electrical and Electronic Engineering, Surgery

View Paper: https://www.semanticscholar.org/paper/f155b7e0a864383cd1fdd33b9ed0053922427044

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In Cell Biology

1. J. Pollard
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Citations in the corpus

• Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. (2015, methodology): “However, they are time consuming, larger amount of cells are required, and chemical gradients cannot be established (Liang et al., 2007; van der Meer et al., 2010).”
• Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In Vitro (2015, methodology): “We next studied MSC exosomes in their ability to induce dermal fibroblast migration during the wound-healing process by using the scratch assay method, an in vitro procedure used to study cell migration [35].”
• ADAMTS-7 Mediates Vascular Smooth Muscle Cell Migration and Neointima Formation in Balloon-Injured Rat Arteries (2009, methodology): “VSMC migration was assessed by scratch-wound assay as described previously.(3)”
• The high mobility group protein HMG20A cooperates with the histone reader PHF14 to modulate TGFβ and Hippo pathways (2022, methodology): “Wound-healing assays for determination of migration were performed as previously described (24).”
• Bayesian Synthetic Likelihood (2017, background): “a confluent monolayer), a ‘scratch’ is made which separates the cells (Liang et al., 2007).”
• An image J plugin for the high throughput image analysis of in vitro scratch wound healing assays (2020, background): “Our results indicate that accurate analyses are likely to be found for variance window radii is between 3 to 25, threshold values in the range of 50 to 150 and percentages of saturated pixels between 0.001 to 0.4.”
• YIA3: ADAMTS7 CLEAVAGE AND VASCULAR SMOOTH MUSCLE CELL MIGRATION IS AFFECTED BY A CORONARY ARTERY DISEASE ASSOCIATED VARIANT (2013, methodology): “Scratch assays were carried out using a previously described method.(11) Migration assays were performed with the use of trans-”
• Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets (2019, methodology): “This research was supported by Wellcome Trust (101067/Z/13/Z), MRC Center grant MR/N022556/1, and NIH grant PO1CA100324 to J.W.P., Department of Defense Breast Cancer Research Program (W81XWH-111-0702) to L.M.C. and J.W.P., Susan G Komen Breast Cancer Foundation (KG111084 and KG110560) to E.S.H. and”
• MicroRNA 21 Promotes Glioma Invasion by Targeting Matrix Metalloproteinase Regulators (2008, methodology): “First, we performed an in vitro scratch motility assay on A172 cells (35).”