OpenSPIM: an open-access light-sheet microscopy platform

By P. Pitrone, Johannes E. Schindelin, L. Stuyvenberg, Stephan Preibisch, Michael Weber, Kevin W. Eliceiri, Jan Huisken, Pavel Tomancak. Nature Methods, 2013

329 citations recorded by the source corpus.

Light sheet microscopy promises to revolutionize developmental biology by enabling live in toto imaging of entire embryos with minimal phototoxicity. We present detailed instructions for building a compact and customizable Selective Plane Illumination Microscopy (SPIM) system. The integrated OpenSPIM hardware and software platform is shared with the scientific community through a public website, thereby making light sheet microscopy accessible for widespread use and optimization to various applications.

Subfields: Biophysics, Structural Biology

View Paper: https://www.semanticscholar.org/paper/f13db2aa3e459add055bd564e4e91f6b1ba1a4da

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biophysics

1. Curtis T. Rueden
2. Kevin W. Eliceiri
3. Johannes E. Schindelin
4. Mark C. Hiner

In Structural Biology

1. Kevin W. Eliceiri
2. Curtis T. Rueden
3. Johannes E. Schindelin

Citations in the corpus

• The ImageJ ecosystem: an open platform for biomedical image analysis (2015, background): “It also proves invaluable as the starting point for new imaging technologies (e.g., the OpenSPIM platform (Pitrone et al., 2013), which makes selective-plane illumination microscopy (SPIM) accessible for everyday use in life science and to develop the technology further).”
• Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis. (2014, unknown): “Improvements in illumination (Kalchmair et al., 2010; Leischner et al., 2009) and image processing (Mertz and Kim, 2010) and technological sharing through open platforms such as OpenSPIM (Pitrone et al., 2013) will also advance the speed and resolution of whole-brain imaging.”