A functional CFTR assay using primary cystic fibrosis intestinal organoids
By J. Dekkers, C. L. Wiegerinck, H. Jonge, I. Bronsveld, H. Janssens, K. W. Groot, A. Brandsma, N. Jong, M. Bijvelds, B. Scholte and 6 more. Nature Medicine, 2013
958 citations recorded by the source corpus.
Subfields: Pulmonary and Respiratory Medicine
View Paper: https://www.semanticscholar.org/paper/ecc66a7026dec9b25016afc07746d9a6ec20e016
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In Pulmonary and Respiratory Medicine
Citations in the corpus
- Surrogate Wnt agonists that phenocopy canonical Wnt/β-catenin signaling (2017, positive): “Intestinal organoids represent a model for personalized cystic fibrosis drug screening 21 .”
- Organogenesis in a dish: Modeling development and disease using organoid technologies (2014, background): “1016/0304-3940(86)90019-4; pmid: 2419803”
- Modeling Development and Disease with Organoids. (2016, methodology): “As a first example, the colon organoid-based CF test (Dekkers et al., 2013) can be read out in weeks after biopsy.”
- Long‐term expanding human airway organoids for disease modeling (2019, positive): “Following opening of the CFTR channel by cAMP-inducing agents (e.g., forskolin), anions and fluid are transported to the orga-noid lumen resulting in rapid organoid swelling (Dekkers et al , 2013), allowing personalized in vitro drug screenings (Dekkers et al , 2016).”
- Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver (2015, positive): “…the technology to allow culturing of human intestinal stem cells (Jung et al., 2011; Sato et al., 2011) and have shown that patient-derived intestinal organoids recapitulate the pathology of hereditary intestinal diseases (Bigorgne et al., 2014; Dekkers et al., 2013; Wiegerinck et al., 2014).”
- Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients. (2013, background): “These numbers are comparable to forskolin-induced surface area increase of wild-type organoids and exceed CFTR rescue capacities obtained with chemical correctors (Dekkers et al., 2013).”
- Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning (2018, methodology): “, 2015), an organoid model of cystic fibrosis for drug screening has been described (Dekkers et al., 2013) and a high-throughput platform for intestinal stem cell niche co-cultures has been developed (Gracz et al.”