A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics*

By Mathias Uhlen, Erik Björling, Charlotta Agaton, C. Szigyarto, B. Amini, E. Andersen, A. Andersson, P. Angelidou, A. Asplund, C. Asplund and 57 more. Molecular & Cellular Proteomics, 2005

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Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, ∼400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.

Subfields: Molecular Biology, Radiology, Nuclear Medicine and Imaging, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/eb69fc3e63086cbf16fa156eef314d187ca0832b

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Citations in the corpus

Reconstruction of Genome-Scale Active Metabolic Networks for 69 Human Cell Types and 16 Cancer Types Using INIT (2012, background): “The INIT (Integrative Network Inference for Tissues) algorithm is formulated as a mixed integer-linear problem (MILP) and is specially tailored to use the evidence from the HPA as input.”
The enemy who sealed the world: effects quarantine due to the COVID-19 on sleep quality, anxiety, and psychological distress in the Italian population (2020, neutral): “For other viruses that use viral receptors different from DPP4, there may also be human tissues where the viral receptor and Fc receptor are both expressed.”
Automated subcellular location determination and high-throughput microscopy. (2007, methodology): “org) has used immunocytochemical staining to obtain images of over 700 monospecific antibodies in 48 normal human tissues and 20 tumors (Uhlen et al., 2005).”
Prediction of the human membrane proteome (2010, methodology): “org) [25, 26], with the predicted membrane protein topology visualized for each method.”
The Human Protein Atlas—a tool for pathology (2008, methodology): “%) an uncertain IHC validation score.”
Gene regulatory network inference in the era of single-cell multi-omics (2023, background): “Alternatively, databases such as The Human Protein Atlas 105,106 can be queried to confirm whether a candidate TF has been previously reported to be present at the protein level in a given tissue or cell type.”
Open Targets Platform: new developments and updates two years on (2018, methodology): “We have enhanced both the data and the visualisation for baseline expression by combining both RNA (16) and protein (31) expression data under a single section entitled ‘RNA and protein baseline expression’.”