Gene essentiality and synthetic lethality in haploid human cells

By V. Blomen, P. Májek, L. T. Jae, J. Bigenzahn, Joppe Nieuwenhuis, J. Staring, R. Sacco, Ferdy R. van Diemen, N. Olk, Alexey Stukalov and 7 more. Science, 2015

809 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e964eec826f48f6bec6e62319655e34764a13335

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In Molecular Biology

Citations in the corpus

High-throughput discovery of novel developmental phenotypes (2016, positive): “Annotations of all human protein-coding genes (Ensembl Genes version 82 15 ), including essential/non-essential status, RVIS 16 , pLI scores (Exome Aggregation Consortium, submitted) and human disease annotations from HGMD 17 and OMIM 18 , were listed in Supplementary Table 8.”
Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens (2017, background): “…for TKOv1), and more of these hits intersect with a list of fitness genes identified in the same cell line, using a comprehensive gene trap screen (Blomen et al. 2015) [n = 1534 for TKOv3 vs. n = 1255 for TKOv1, out of n = 2352 fitness genes at 5% FDR identified in Blomen et al. (2015); Figure…”
Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq. (2016, neutral): “This may result in false-negative and false-positive results, as perturbations may change cell compositions and states that are difﬁcult to decompose without more comprehensive phenotyping (Blomen et al., 2015; Scholl et al., 2009; Tong et al., 2001; Wang et al., 2013).”
RACK1 on and off the ribosome (2019, background): “While much less is known about RP essentiality in mammals, preliminary work suggests that several RPs are non-essential within mammalian cell lines (Blomen et al. 2015) (Supp.”
A deep proteome and transcriptome abundance atlas of 29 healthy human tissues (2018, methodology): “Essential genes (n=583) were assembled from three human essential genes studies using CRISPR-Cas9 and retroviral gene-trap genetic screens (T. Wang et al. 2015; Hart et al. 2015; Blomen et al. 2015).”
Uncovering the essential genome of the human malaria parasite Plasmodium falciparum by saturation mutagenesis (2018, positive): “falciparum, as well as data from recent large-scale mutagenesis studies of apicomplexan genomes (6, 18, 27-29) to evaluate the conservation of gene essentiality across the organisms’ lineages.”
Fat(al) attraction: Picornaviruses Usurp Lipid Transfer at Membrane Contact Sites to Create Replication Organelles (2016, background): “A very recent study discovered C10orf76 as a novel enterovirus host factor [55].”