Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq.

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Predicting transcriptional outcomes of novel multigene perturbations with GEARS (2023, positive): “For example, two genes that independently cause a minor loss in cell growth could synergistically interact with one another following combinatorial perturbation to cause cell death.”
Pooled CRISPR screening with single-cell transcriptome read-out (2017, methodology): “While this paper was in review 13 , two alternative methods – Perturb-seq and CRISP-seq – were published that use transcribed barcodes for single-cell CRISPR screening 14–16 .”
A Unique Microglia Type Associated with Restricting Development of Alzheimer's Disease. (2017, neutral): “Single-cell analysis can further identify potential markers, pathways, and regulatory factors, promoting testable hypotheses to elucidatemolecular mechanisms of immune regulation in AD (Jaitin et al., 2016; Matcovitch-Natan et al., 2016; Paul et al., 2015).”
Exploring genetic interaction manifolds constructed from rich single-cell phenotypes (2019, methodology): “For example, Perturb-seq pairs CRISPR-based screens with single-cell RNA sequencing ( 5 – 8 ).”
The Human Cell Atlas (2017, background): “…variation among cells to engineered perturbations, by using pooled CRISPR libraries to manipulate genes and reading out both the perturbation and its effects on cellular phenotype in single cells—for example, by single-cell RNA-Seq (Adamson et al., 2016; Dixit et al., 2016; Jaitin et al., 2016).”