Proteomics: the move to mixtures.

By Junmin Peng, Steven P. Gygi. Journal of Mass Spectrometry, 2001

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A conserved MST-FOXO signaling pathway mediates oxidative-stress responses and extends life span. (2006, positive): “Coomassie blue-stained bands corresponding to FOXO3 and FOXO1 (unphosphorylated or phosphorylated with MST1) were digested with trypsin as described (Peng and Gygi, 2001).”
A proteomics approach to understanding protein ubiquitination (2003, methodology): “During the elution, peptide ions were constantly detected and sequenced (fragmented) in an automated fashion, with one peptide being sequenced on average every 2”
Comparative evaluation of mass spectrometry platforms used in large-scale proteomics investigations (2005, methodology): “5-cm × 10-cm slices were subjected to in-gel tryptic digestio”
Proteomic analysis of post-translational modifications (2003, background): “In an elegant experiment, yeast ubiquitin was replaced by a histidine-tagged version, allowing selective purification and identification of the ubiquitinated proteome (ref”
Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15 (2017, neutral): “6 mm C18 spherical silica beads into a fused silica capillary (100 mm inner diameter × ~30 cm length) with a flamedrawn tip (49).”