PCR inhibitors – occurrence, properties and removal

By C. Schrader, A. Schielke, L. Ellerbroek, R. Johne. Journal of Applied Microbiology, 2012

1,890 citations recorded by the source corpus.

Subfields: Infectious Diseases, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e4652b53ab0b694afa172075d4f26c9d5200bba7

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Infectious Diseases

1. James J. Collins

In Molecular Biology

1. C. Turner
2. M. Barnes
3. Alexander A. Green
4. C. Jerde
5. Tom Ferrante
6. Keith Pardee
7. James J. Collins
8. N. Ali

Citations in the corpus

• Particle size distribution and optimal capture of aqueous macrobial eDNA (2014, background): “Finally, increased understanding of the PSD of macrobial eDNA may informmitigation of one of themost troublesome issues associated with genetic testing for low-level DNA in environmental samples: sample interference (Schrader et al. 2012).”
• Experimental observations on the decay of environmental DNA from bighead and silver carps (2017, background): “…used in these experiments may have held some level of co-occurring PCR inhibitors, including those typically associated with fecal material (bile salts and complex polysaccharides; reviewed in Schrader et al. 2012) and apparent spikes of eDNA could result from breakdown of PCR inhibitors over time.”
• Application of high-throughput sequencing (HTS) metabarcoding to diatom biomonitoring: Do DNA extraction methods matter? (2017, background): “…of compounds that inhibit or decrease the sensitivity of PCR. PCR-inhibitor molecules in extracted DNA can come from residual compounds of the extraction process (e.g., ethanol) or from molecules that are co-extracted with DNA (e.g., protein, polysaccharides, humic acids) (Schrader et al. 2012).”
• Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics (2017, methodology): “(5) Chelex extraction Quick and simple protocol; no use of hazardous chemicals Low purity of nucleic acids [35, 36]”
• Surveillance of wild birds for avian influenza virus in Korea (2013, neutral): “최근에는 면봉의 재질에 따라 PCR 저해물질을 포 함 할 수 있다는 가설이 제시되어 나무 면봉이 아닌 플라스 틱 면봉을 사용할 것이 추천되고 있으나, 실제로 유전자 검 출 결과에는 큰 영향을 나타내지 않는 것으로 보고되고 있 다 [13].”
• Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components. (2016, positive): “As a result, accurate diagnosis re- quires nucleic acid-based detection methods, such as PCR and isothermal nucleic acid amplification (Lanciotti et al., 2008; de M Campos et al., 2016; Tappe et al., 2014; Zammarchi et al., 2015).”