Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.
By Keith Pardee, Alexander A. Green, Melissa K. Takahashi, D. Braff, G. Lambert, Jeong Wook Lee, Tom Ferrante, D. Ma, Nina M. Donghia, Melina Fan and 6 more. Cell, 2016
1,274 citations recorded by the source corpus.
The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, we detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled with a novel CRISPR/Cas9-based module, our sensors can discriminate between viral strains with single-base resolution. We successfully demonstrate a simple, field-ready sample-processing workflow and detect Zika virus from the plasma of a viremic macaque. Our freeze-dried biomolecular platform resolves important practical limitations to the deployment of molecular diagnostics in the field and demonstrates how synthetic biology can be used to develop diagnostic tools for confronting global health crises. PAPERCLIP.
Subfields: Biomedical Engineering, Molecular Biology, Public Health, Environmental and Occupational Health
View Paper: https://www.semanticscholar.org/paper/038dd146c95ead2f1b7e88b3ee7310b5d065e314
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Citations in the corpus
- CRISPR/Cas Systems towards Next-Generation Biosensing. (2019, methodology): “Deactivated Cas9 effectors fused with split HRP protein have been engineered for activating the colorimetric change of TMB after recognizing and binding to the target. dCas9, Nuclease-deactivated Cas9; dsDNA, doublestranded DNA; HRP, horseradish peroxidase; NASBACC, nucleic acid sequence-based ampli”
- Face Masks in the New COVID-19 Normal: Materials, Testing, and Perspectives (2020, background): “The soaking of contaminated masks in 70% ethanol was recently reported by Smith et al. to cause impairment in mask function within 30 minutes although no SARS-CoV-2 viral RNA could be cultured [116].”
- The growing impact of lyophilized cell-free protein expression systems (2017, methodology): “This cell-free platform was lyophilized into a paper support, and when reconstituted, allowed portable virus detection.(28,29) We recently reported the on-demand production of the cytotoxic cancer therapeutic Onconase”
- Nucleic acid detection with CRISPR-Cas13a/C2c2 (2017, positive): “To explore the potential use of SHERLOCK in the field with paper-spotting and lyophilization (1), we first demonstrated that Cas13a-crRNA complexes lyophilized and subsequently rehydrated (20) could detect 20 fM of non-amplified ssRNA 1 (fig.”