Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics
By N. Ali, R. Rampazzo, A. Costa, Marco A. Krieger. BioMed Research International, 2017
322 citations recorded by the source corpus.
Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities that influence their use, especially in point-of-care diagnostics (POC-Dx). POC-Dx is a new approach aiming to replace sophisticated analytical machinery with microanalytical systems, able to be used near the patient, at the point of care or point of need. Although notable efforts have been made, a simple and effective extraction method is still a major challenge for widespread use of POC-Dx. In this review, we dissected the working principle of each of the most common NAE methods, overviewing their advantages and disadvantages, as well their potential for integration in POC-Dx systems. At present, it seems difficult, if not impossible, to establish a procedure which can be universally applied to POC-Dx. We also discuss the effects of the NAE chemicals upon the main plastic polymers used to mass produce POC-Dx systems. We end our review discussing the limitations and challenges that should guide the quest for an efficient extraction method that can be integrated in a POC-Dx system.
Subfields: Biomedical Engineering, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/b09be3a07b18a99c79463f94df825118acf23685
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Biomedical Engineering
In Molecular Biology
Citations in the corpus
- The Evolution of DNA Extraction Methods (2020, methodology): “The principal behind DNA extraction consists of the following steps: (1) disruption of cytoplasmic and nuclear membranes; (2) separation and purification of DNA from other components of the cell lysate such as lipids, proteins, and other nucleic acids; and (3) concentration and purification of DNA [”