Genes required for mycobacterial growth defined by high density mutagenesis

By Christopher M. Sassetti, D. Boyd, Eric J. Rubin. Molecular Microbiology, 2003

2,577 citations recorded by the source corpus.

Subfields: Ecology, Epidemiology, Infectious Diseases

View Paper: https://www.semanticscholar.org/paper/e45efcd480a22e65f1607ea7b6dc93e3dee81e6d

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In Ecology

Christopher M. Sassetti

In Epidemiology

In Infectious Diseases

Citations in the corpus

High-Resolution Phenotypic Profiling Defines Genes Essential for Mycobacterial Growth and Cholesterol Catabolism (2011, background): “About 15% of the Mtb genome, or 614 genes, were previously predicted to be required for optimal growth in vitro using microarray hybridization to map insertions sites [6].”
DEG 10, an update of the database of essential genes that includes both protein-coding genes and noncoding genomic elements (2013, methodology): “Mycobacterium tuberculosis 614 Transposon mutagenesis followed by hybridization (TraSH) Yes (58)”
Pathway to Synthesis and Processing of Mycolic Acids in Mycobacterium tuberculosis (2005, background): “tuberculosis strain H37Rv transposon mutant library grown on agar plates (87).”
Silencing Essential Protein Secretion in Mycobacterium smegmatis by Using Tetracycline Repressors (2007, background): “tuberculosis on agar plates have recently been identified by genome-wide mutagenesis (24, 37).”
PathogenFinder - Distinguishing Friend from Foe Using Bacterial Whole Genome Sequence Data (2013, background): “The existence of diseases caused by bacteria that cannot grow in pure culture medium [4,5], the discovery of polymicrobial diseases [6], the role of metagenomic microbiota in chronic diseases [7], and last but not least, the discovery of Horizontal Gene Transfer (HGT) responsible for the swapping of”
Mycobacterial persistence requires the utilization of host cholesterol (2008, background): “We reported that Mce4 was specifically required for bacterial survival during the later stages of infection in the mouse spleen (21, 29).”
Probabilistic integrative modeling of genome-scale metabolic and regulatory networks in Escherichia coli and Mycobacterium tuberculosis (2010, background): “A drug that targets the gene Rv0485 would also affect its regulatory targets—glmU, glmS, and nagA, which were all found to be essential (30).”
Biological roles of glycans (2016, background): “Knockouts of these genes in mycobacteria are lethal, as is the case with some other glycosyltransferases (435).”