De novo generation of neuronal cells from the adult mouse brain.

By L. Richards, T. Kilpatrick, P. Bartlett. 1992

Cells of neuronal morphology, expressing the 150- and 200-kDa neurofilament proteins, were generated in vitro from populations of neural cells dissociated from adult (greater than 60-day-old) mouse brain. Most of these neurons arose from dividing precursors, as demonstrated by the incorporation of [3H]thymidine during the culture period and autoradiography. Neuronal production was optimal under the conditions in which precursors were initially stimulated with basic fibroblast growth factor and then exposed to medium conditioned by an astrocytic cell line, Ast-1, in serum-free medium. Few, if any, neurons arose in control cultures or in cultures kept in serum and fibroblast growth factor. These results suggest that neuronal precursors exist in the adult mammalian brain, but they require discrete epigenetic signals for their proliferation and differentiation.

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Citations in the corpus

Adult Neurogenesis in the Mammalian Brain: Significant Answers and Significant Questions (2011, background): “Multipotent neural stem cells were later derived from the adult mammalian brain (Reynolds and Weiss, 1992; Richards et al., 1992).”
Regulation of distinct AMPA receptor phosphorylation sites during bidirectional synaptic plasticity (2000, background): “Note that single-channel conductance increases with LTP 24 .”
Peripheral Infusion of IGF-I Selectively Induces Neurogenesis in the Adult Rat Hippocampus (2000, background): “However, self-renewing cells with multilineage potential, designated stem cells or progenitor cells, have been identified in the adult mammalian brain (Reynolds and Weiss, 1992; Richards et al., 1992; Craig et al., 1996; Suhonen et al., 1996; McKay, 1997; Temple and Alvarez-Buylla, 1999).”
Stem and progenitor cells: origins, phenotypes, lineage commitments, and transdifferentiations. (2001, background): “…rodent fetal brain or adult SVZ or adult DG cells can grow in vitro in the presence of defined factors such as bFGF and EGF (Reynolds & Weiss 1992, Richards et al. 1992, Temple & Alvarez-Buylla 1999, Temple & Davis 1994, Gritti et al. 1996, Johe et al. 1996, Kalyani et al. 1997, Mayer-Proschel et…”
Neurogenesis in the Adult Brain (2002, background): “This was first shown from the subventricular zone (Reynolds and Weiss, 1992; Richards et al., 1992) and then in the dentate gyrus of the hippocampus (Gage et al., 1995; Palmer et al., 1997), and in most structures of the brain examined (Palmer et al., 1995; Shihabuddin et al., 2000), even where no…”
Purification of a pluripotent neural stem cell from the adult mouse brain (2001, background): “Recently, the possibility has been raised that bone marrow cells have the potential to give rise to neurons in vivo”
Complexity in biomaterials for tissue engineering. (2009, background): “The discovery of other types of cell with multilineage potential has since followed, including neural stem cells from the brain, and mesenchymal stem cells, which can differentiate into bone, fat, cartilage and muscle cell”
The Adult Substantia Nigra Contains Progenitor Cells with Neurogenic Potential (2002, background): “Isolated progenitor cells were cultured in the presence of either FGF2, which has been described as a necessary mitogen for maintaining multipotent progenitor cells in vitro (Richards et al., 1992; Vescovi et al., 1993; Palmer et al., 1995; Shihabuddin et al., 1997), or FGF8, which is a mitogen for ”
Adult neurogenesis and neural stem cells of the central nervous system in mammals (2002, background): “NPCs have since been isolated from diverse areas of the adult CNS: in mouse brain (Richards et al., 1992); in the SVZ of mouse (Lois and Alvarez-Buylla, 1993; Morshead et al., 1994), rat (Palmer et al., 1995) and human (Kirschenbaum et al., 1994; Pincus et al., 1998; Kukekov et al., 1999;…”
In vivo fate mapping and expression analysis reveals molecular hallmarks of prospectively isolated adult neural stem cells. (2010, background): “744 Cell Stem Cell 7, 744–758, December 3, 2010 a2010 Elsevier Inc riched as neurospheres in vitro (Reynolds et al., 1992; Richards et al., 1992).”
Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS (1999, background): “…et al., 1994), and many groups have noted that FGF-2 is a necessary mitogen for maintaining proliferative multipotent precursors in vitro (Richards et al., 1992; Vescovi et al., 1993; Kilpatrick and Bartlett, 1995; Gritti et al., 1999; Tropepe et al., 1999), but little is known regarding…”
Delayed Transplantation of Adult Neural Precursor Cells Promotes Remyelination and Functional Neurological Recovery after Spinal Cord Injury (2006, unknown): “…stem cells (ESCs), adult neural precursor cells (NPCs) have shown an extensive capacity for self-renewal and multipotency in vitro (Reynolds and Weiss, 1992; Richards et al., 1992), and, importantly, they obviate the potential ethical issues surrounding the use of ESCs for regenerative therapies.”