The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

By P. Chomczyński, N. Sacchi. Nature Protocols, 2006

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DNA, RNA, and Protein Extraction: The Past and The Present (2009, methodology): “The principle of this single-step technique is that RNA is separated from DNA after extraction with acidic solution consisting guanidinium thiocyanate, sodium acetate, phenol, and chloroform [13].”
Touchdown PCR for increased specificity and sensitivity in PCR amplification (2008, methodology): “cDNA sample preparation requires RNA isolation followed by reverse transcription; the acid guanidinium metho”
Transcriptomics technologies (2017, background): “Although biological systems are incredibly diverse, RNA extraction techniques are broadly similar and involve the following: mechanical disruption of cells or tissues, disruption of RNase with chaotropic salts [43], disruption of macromolecules and nucleotide complexes, separation of RNA from undesi”
GLK Transcription Factors Coordinate Expression of the Photosynthetic Apparatus in Arabidopsis[W][OA] (2009, methodology): “extraction (Chomczynski and Sacchi, 2006), except for the data shown in”
A Broad Set of Chromatin Factors Influences Splicing (2016, methodology): “RNA purificationand RT-PCR The RNA samples were prepared as described [44] and the cDNA libraries were synthesized with M-MLV reverse transcriptase using oligo dT priming or random primers.”