Touchdown PCR for increased specificity and sensitivity in PCR amplification

By D. Korbie, John S. Mattick. Nature Protocols, 2008

637 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/db66052240e96c69a2824202058d8f13b94f1e78

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In Molecular Biology

1. P. Ozias‐Akins
2. Y. Chu

Citations in the corpus

• Recombination is suppressed in an alien introgression in peanut harboring Rma, a dominant root-knot nematode resistance gene (2010, methodology): “using a standard touchdown PCR protocol (Korbie and Mattick 2008) applied to a 20 ll solution containing 2.”