DNA, RNA, and Protein Extraction: The Past and The Present
By S. Tan, B. C. Yiap. Journal of Biomedicine and Biotechnology, 2009
776 citations recorded by the source corpus.
Extraction of DNA, RNA, and protein is the basic method used in molecular biology. These biomolecules can be isolated from any biological material for subsequent downstream processes, analytical, or preparative purposes. In the past, the process of extraction and purification of nucleic acids used to be complicated, time-consuming, labor-intensive, and limited in terms of overall throughput. Currently, there are many specialized methods that can be used to extract pure biomolecules, such as solution-based and column-based protocols. Manual method has certainly come a long way over time with various commercial offerings which included complete kits containing most of the components needed to isolate nucleic acid, but most of them require repeated centrifugation steps, followed by removal of supernatants depending on the type of specimen and additional mechanical treatment. Automated systems designed for medium-to-large laboratories have grown in demand over recent years. It is an alternative to labor-intensive manual methods. The technology should allow a high throughput of samples; the yield, purity, reproducibility, and scalability of the biomolecules as well as the speed, accuracy, and reliability of the assay should be maximal, while minimizing the risk of cross-contamination.
Subfields: Ecology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/a1a159240fc02a67d816f9e1ebced479da5f7f8a
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Citations in the corpus
- The Evolution of DNA Extraction Methods (2020, background): “Consequently, DNA is eluted from the column faster than the smaller molecules [18,19].”
- Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics (2017, methodology): “Solid-phase extraction (SPE) is one of the most efficient NAE techniques available in the market [1, 5].”