A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

By P. Kralik, Matteo Ricchi. Frontiers in Microbiology, 2017

824 citations recorded by the source corpus.

Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety. Although the concept of PCR is relatively simple, there are specific issues in qPCR that developers and users of this technology must bear in mind. These include the use of correct terminology and definitions, understanding of the principle of PCR, difficulties with interpretation and presentation of data, the limitations of qPCR in different areas of microbial diagnostics and parameters important for the description of qPCR performance. It is not our intention in this review to describe every single aspect of qPCR design, optimization, and validation; however, it is our hope that this basic guide will help to orient beginners and users of qPCR in the use of this powerful technique.

Subfields: Food Science, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/b2ad25bea813474fd47ddbc16ae49e9a2e6ec36c

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Food Science

1. J. V. van Loon
2. D. Oonincx
3. A. van Huis

In Molecular Biology

1. D. Oonincx
2. A. van Huis
3. J. Tomberlin

Citations in the corpus

• Standardisation of quantitative resource conversion studies with black soldier fly larvae (2020, methodology): “As with isolation, PCR controls should be included with every PCR run and are also necessary for monitoring aspects of the PCR reaction such as reagent activity, cycling parameters, potential contamination, and for data interpretation (Kralik and Ricchi, 2017).”