The new frontier of genome engineering with CRISPR-Cas9

By Jennifer A. Doudna, E. Charpentier. 2014

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Citations in the corpus

Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq. (2016, positive): “Current CRISPR/Cas methods are used either for individual gene KOs or for pooled screens (Doudna and Charpentier, 2014; Hsu et al., 2014).”
Genetics and genomics of moso bamboo (Phyllostachys edulis): Current status, future challenges, and biotechnological opportunities toward a sustainable bamboo industry (2020, background): “Latest advancements in gene editing technologies have found increasing use of clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated endonuclease (Cas9) system in several crop species (Doudna & Charpentier, 2014).”
Identifying and visualizing functional PAM diversity across CRISPR-Cas systems (2016, background): “…Repeats) and their associated Cas (CRISPR associated) proteins form widespread adaptive immune systems in prokaryotes that have been harnessed as ubiquitous tools in biotechnology and medicine (Barrangou et al., 2007; Doudna and Charpentier, 2014; Hsu et al., 2014; van der Oost et al., 2014).”
CRISPR/Cas9 in Genome Editing and Beyond. (2016, methodology): “The Cas9-mediated gene-editing system has been broadly used in reverse genetics studies to understand the role of specific genes, for disease modeling, and for demonstrating new therapeutic schemes in a number of models of genetic and infectious diseases (9, 10, 94).”
Genome Engineering Using the CRISPR Cas9 System (2019, background): “The CRISPR-Cas9 system which serves as an endonuclease employed a guide RNA which is the integration of CrRNA and TracrRNA and bind to the target through base pairing [8,14].”
DeepCRISPR: optimized CRISPR guide RNA design by deep learning (2018, background): “Such a CRISPR-Cas9 endonuclease system permits genome editing at nucleotide resolution [3, 4], while a major challenge for its effective application is to accurately predict the sgRNA on-target knockout efficacy and off-target (OT) profile beforehand.”
Generation of knock-in primary human T cells using Cas9 ribonucleoproteins (2015, methodology): “T CRISPR/Cas9 system has been used increasingly to edit mammalian germline sequence and cell lines (1, 2).”
CRISPR-Cas12a: Functional overview and applications (2020, background): “Additionally, Cas12a also possesses an RNA processing site [1,9,24,31,34,37].”