Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

By Kathrin Schumann, Steven Lin, E. Boyer, Dimitre R. Simeonov, Meena Subramaniam, Rachel E. Gate, Genevieve E. Haliburton, Chun Jimmie Ye, J. Bluestone, Jennifer A. Doudna and 1 more. Proceedings of the National Academy of Sciences of the United States of America, 2015

675 citations recorded by the source corpus.

Subfields: Molecular Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/8ff28a21bb88c381f81d9cb63b5c7009c5251447

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. C. June
2. Jerilyn A. Timlin
3. J. Carney
4. J. C. Harper
5. Wendell A. Lim
6. Christopher A. Lino

In Oncology

1. C. June
2. Wendell A. Lim

Citations in the corpus

• Delivering CRISPR: a review of the challenges and approaches (2018, background): “Electroporation for delivery of Cas9:sgRNA ribonucleoprotein (RNP) to primary human cells has also been reported (Kim et al., 2014; Schumann et al., 2015).”
• The Principles of Engineering Immune Cells to Treat Cancer (2017, background): “Finally, several recent efforts have been made to use CRISPR genome editing to remove the PD-1 receptor from T cells, rendering them non-responsive to PD-L1-mediated suppression (Ren et al., 2016; Schumann et al., 2015).”