Jasmonate perception by inositol phosphate-potentiated COI1-JAZ co-receptor

By Laura B. Sheard, Xu Tan, Haibin Mao, John Withers, Gili Ben-Nissan, T. Hinds, Yuichi Kobayashi, F. Hsu, M. Sharon, J. Browse and 4 more. Nature, 2010

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Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved α-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.

Subfields: Insect Science, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/dfa8463fc9333814ae6db30d49639d69acc04f70

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Citations in the corpus

Jasmonates: biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany. (2013, methodology): “This hexapeptide within the Jas domain represents the conserved degron motif, and is required for closing off the binding pocket of JA-Ile within the receptor complex (Sheard et al., 2010).”
Jasmonate Regulates the INDUCER OF CBF EXPRESSION–C-REPEAT BINDING FACTOR/DRE BINDING FACTOR1 Cascade and Freezing Tolerance in Arabidopsis[W] (2013, background): “Jasmonate is perceived by the F-box protein CORONATINE INSENSITIVE1 (COI1), which subsequently facilitates the degradation of JAZ proteins via the SCFCOI1-26S proteasome pathway (Xu et al., 2002; Chini et al., 2007; Thines et al., 2007; Yan et al., 2009; Sheard et al., 2010).”
Hormonal modulation of plant immunity. (2012, background): “Also, nymphs of PRRsPM TGA3 ARR2 SID2 EIN3/ EIL1 SCFTIR1/AFB AvrRpt2 AvrRpt2 miR393 AvrPtoB HopI1 HopI1 HSP70 SCFCOI1 DELLAs AvrPtoB PAMP TT SS PAMP COR COR COR GA CK ET Auxin Auxin response ABA JA response ABA response ?”
Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome. (2017, background): “However, these algal F-box orthologs do not possess conserved key residues for interaction with corresponding land plant ligands (JA-Ile/JAZ [Sheard et al., 2010] and auxin and AUX/IAA [Tan et al., 2007]; Figure S2), suggesting that they are neither auxin nor JA receptors.”
Costs and benefits of hormone-regulated plant defences (2013, background): “, 1998), as it is part of the JA receptor complex (Yan et al., 2009; Sheard et al., 2010).”
Jasmonates counter plant stress: A Review (2015, neutral): “in SCF complex (Yan et al., 2009; Sheard et al., 2010).”
Arabidopsis MYC2 Interacts with DELLA Proteins in Regulating Sesquiterpene Synthase Gene Expression[W][OA] (2012, background): “The JAZ family proteins function as repressors of the JA signaling pathway, and a recent structural and pharmacological study showed that COI1 and JAZ form a coreceptor complex (Sheard et al., 2010).”
Jasmonic Acid Signaling Pathway in Plants (2019, background): “proposed different views on JAs receptors through the analysis of crystal structure and confirmed that the COI1–JAZ complex is a high-affinity receptor for the bioactive JA-Ile; that is, COI1 and JAZ are coreceptors of JA signaling [41].”
The Arabidopsis bHLH Transcription Factors MYC3 and MYC4 Are Targets of JAZ Repressors and Act Additively with MYC2 in the Activation of Jasmonate Responses[C][W] (2011, background): “A genetic screen for mutants altered in Trp metabolism identified atr2D, a dominant mutant in MYC3 (Smolen et al., 2002).”
The Jasmonate-ZIM-Domain Proteins Interact with the WD-Repeat/bHLH/MYB Complexes to Regulate Jasmonate-Mediated Anthocyanin Accumulation and Trichome Initiation in Arabidopsis thaliana[C][W] (2011, background): “…the expression of late anthocyanin biosynthetic genes, including NADPH-dependent dihydroflavonol reductase (DFR), leucoanthocyanidin dioxygenase (LDOX), and UDP-Glc:flavonoid 3-Oglucosyltransferase (UF3GT) (Dooner et al., 1991; Kubasek et al., 1992; Shirley et al., 1995; Gonzalez et al., 2008).”
Jasmonate Biosynthesis, Perception and Function in Plant Development and Stress Responses (2013, background): “JA initiates signaling process upon formation of a SCFCOI1-JA-Ile-JAZ ternary complex (JAZ: jasmonate ZIM-domain protein; Sheard et al., 2010), in which the JAZ repressors are ubiquitinated and subsequently degraded to release transcription factors, e.”