Arabidopsis MYC2 Interacts with DELLA Proteins in Regulating Sesquiterpene Synthase Gene Expression[W][OA]

By G. Hong, Xue-Yi Xue, Ying-Bo Mao, Ling-Jian Wang, Xiao-Ya Chen. The Plant Cell, 2012

611 citations recorded by the source corpus.

This work examines the regulation of two sesquiterpene synthases and shows that gibberellin and jasmonate jointly regulate the biosynthesis of sesquiterpenes through the transcription factor MYC2, and the gibberellin signal is transduced to this secondary metabolism pathway through a DELLA–MYC2 interaction Arabidopsis thaliana flowers emit volatile terpenes, which may function in plant–insect interactions. Here, we report that Arabidopsis MYC2, a basic helix-loop-helix transcription factor, directly binds to promoters of the sesquiterpene synthase genes TPS21 and TPS11 and activates their expression. Expression of TPS21 and TPS11 can be induced by the phytohormones gibberellin (GA) and jasmonate (JA), and both inductions require MYC2. The induction of TPS21 and TPS11 results in increased emission of sesquiterpene, especially (E)-β-caryophyllene. DELLAs, the GA signaling repressors, negatively affect sesquiterpene biosynthesis, as the sesquiterpene synthase genes were repressed in plants overaccumulating REPRESSOR OF GA1-3 (RGA), one of the Arabidopsis DELLAs, and upregulated in a penta DELLA-deficient mutant. Yeast two-hybrid and coimmunoprecipitation assays demonstrated that DELLAs, represented by RGA, directly interact with MYC2. In yeast cells, the N terminus of MYC2 was responsible for binding to RGA. MYC2 has been proposed as a major mediator of JA signaling and crosstalk with abscisic acid, ethylene, and light signaling pathways. Our results demonstrate that MYC2 is also connected to GA signaling in regulating a subset of genes. In Arabidopsis inflorescences, it integrates both GA and JA signals into transcriptional regulation of sesquiterpene synthase genes and promotes sesquiterpene production.

Subfields: Insect Science, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/02e98054d26679f58e24ff3e63e0564cffc40937

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Citations in the corpus

Gibberellin signaling in plants (2013, background): “More recently, DELLAs have been shown to interact with and inhibit the activity of numerous transcription regulators (Cheminant et al., 2011; Feurtado et al., 2011; Josse et al., 2011; Hong et al., 2012; An et al., 2012; Zhang et al., 2011).”
Abscisic Acid Synthesis and Response (2013, background): “However, MYC2 is also involved in mediating cross-talk with jasmonate, ethylene, gibberellin, and light signaling, so is hardly specific for ABA response (Hong et al., 2012).”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “The JA-responsive bHLH transcription factor AtMYC2 extensively cross-talks with JA, ethylene, ABA, and GA signalling pathways (Hong et al., 2012).”
Jasmonates: biosynthesis, metabolism, and signaling by proteins activating and repressing transcription (2016, background): “Both DELLA repressors in the GA pathway and JAZs interact with MYC2 to repress sesquiterpene biosynthesis in flowers (Hong et al., 2012).”
Growth-defense tradeoffs in plants: a balancing act to optimize fitness. (2014, neutral): “Conversely, GA has also been demonstrated to have a positive effect on some JA-mediated traits such as sesquiterpene synthase gene expression (Hong et al., 2012).”