Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects
By Bin Shen, Wensheng Zhang, Jun Zhang, Jiankui Zhou, Jianying Wang, Li Chen, Lu Wang, Alex Hodgkins, V. Iyer, Xingxu Huang and 1 more. Nature Methods, 2014
835 citations recorded by the source corpus.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/df948de5d74e982c8d0ffcf6cf1207cc6b44d1c4
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In Molecular Biology
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- Bing Ren
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Citations in the corpus
- The new frontier of genome engineering with CRISPR-Cas9 (2014, positive): “…a double-nicking approach consisting of using the nickase variant of Cas9 with a pair of offset sgRNAs properly positioned on the target DNA ( 146 – 148 ); an sgRNA-guided dCas9 fused to the FokI nuclease where two fused dCas9-FokI monomers ( 149 , 150 ); and shorter sgRNAs truncated by two or…”
- CRISPR Inversion of CTCF Sites Alters Genome Topology and Enhancer/Promoter Function (2015, positive): “1 or pGL3-U6-sgRNA-PGKPuro plasmid (Chang et al., 2013; Cong et al., 2013; Mali et al., 2013; Shen et al., 2014; Li et al., 2015) with appropriate primers (Table S7).”
- CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes (2015, methodology): “1B) (Shen et al., 2014).”
- Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018, methodology): “This method is applicable for genome editing of any model organism with minimal off-target effects [27, 28].”
- A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, background): “An alternative approach to achieving relatively large deletions is by using paired Cas9 nickases (Ran et al., 2013; Ren et al., 2014; Schiml et al., 2014; Shen et al., 2014).”
- The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “This strategy can yield precise genome modifications with efficiencies comparable to the unmodified enzyme, butwith 50- to 1500-fold greater specificity in human and mouse cells (Cho et al., 2014; Duda et al., 2014;Mali et al., 2013; Ran et al., 2013; Shen et al., 2014) and Arabidopsis (Fauser et al”