Identification of in vivo DNA targets of chromatin proteins using tethered Dam methyltransferase

By B. van Steensel, Steven Henikoff. Nature Biotechnology, 2000

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The genomic landscape of the somatic linker histone subtypes H1.1 to H1.5 in human cells. (2013, background): “It is based on the expression of a protein of interest fused to Dam (van Steensel and Henikoff, 2000), a bacterial methyltransferase that specifically methylates adenines within GATC sequences (Greil et al., 2006).”
Computational prediction of transcription-factor binding site locations (2003, methodology): “Another recently developed method that takes advantage of DNA microarrays for the identification of TFBSs in vivo uses TFs tethered to DNA adenine methyl-transferase (Dam) [19,20], resulting in DNA methylation near sites bound by the TF-Dam fusion protein [19,20].”
Single-cell dynamics of genome-nuclear lamina interactions. (2013, methodology): “…genome-NL interactions in single cells builds upon the DamID technology, in which genomic regions that are in molecular contact with a nuclear protein of interest are tagged in vivo with adenine-6-methylation (m6A), a modification unknown to higher eukaryotes (van Steensel and Henikoff, 2000).”
A decade of 3C technologies: insights into nuclear organization. (2012, methodology): “DamID (van Steensel and Henikoff 2000) is one such method that, when directed to proteins of the nuclear lamina, provides maps of chromatin associated with the nuclear periphery (Pickersgill et al. 2006).”