Quantitative Analysis of Small-Subunit rRNA Genes in Mixed Microbial Populations via 5′-Nuclease Assays

By Marcelino T. Suzuki, L. T. Taylor, E. Delong. Applied and Environmental Microbiology, 2000

1,197 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/de6563c9eff74efcd3ff3e0e289c4895cfe73088

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Citations in the corpus

The use of quantitative polymerase chain reaction for the detection and enumeration of the harmful alga Aureococcus anophagefferens in environmental samples along the United States East Coast (2003, methodology): “Statistical properties—qPCR is sensitive over 4 orders of magnitude, in agreement with the range reported by Suzuki et al. (2000).”
Influence of the Amazon River plume on distributions of free‐living and symbiotic cyanobacteria in the western tropical north Atlantic Ocean (2007, methodology): “Recently, several real-time (quantitative) polymerase chain reaction (PCR) assays have been developed and successfully used in a variety of applications, including estimating the abundance of specific planktonic microbial populations (Suzuki et al. 2000; Short et al. 2004; Church et al. 2005a).”
Mapping of picoeucaryotes in marine ecosystems with quantitative PCR of the 18S rRNA gene. (2005, methodology): “[31] using linearized plasmids containing cloned 18S rDNA genes.”
Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction. (2005, background): “…(Reischl et al., 2002; Witt- B 2005 Wiley Periodicals, Inc. Correspondence to: S. Hwang wer et al., 2001) allows rapid, highly sensitive detection of microbial DNA, thus indicating the presence of a target microorganism or group of microorganisms (Nadkarni et al., 2002; Suzuki et al., 2000).”
Optimization of DNA extraction for quantitative marine bacterioplankton community analysis (2004, methodology): “16S rDNA was quantified by real-time PCR using the TaqMan (Applied Biosystems) bacteria-specific BACT2 primer/probe set developed by Suzuki et al. (2000): BACT1369F (1000 nM, final con.)”