Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction.

By Youngseob Yu, Changsoo Lee, Jaai Kim, Seokhwan Hwang. Biotechnology and Bioengineering, 2005

1,577 citations recorded by the source corpus.

Subfields: Building and Construction, Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/364ab0756a8df45c83a75745e5e121155aad5da5

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Building and Construction

In Ecology

In Molecular Biology

Citations in the corpus

Biogas production: current state and perspectives (2009, background): “With molecular techniques, more information can be received about the community structures in anaerobic processes (Elferink et al. 1998; Yu et al. 2005; Karakashev et al. 2005; Klocke et al. 2008 ).”
Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli. (2006, methodology): “Absolute quantification determines the exact copy concentration of target gene by relating the CT value to a standard curve ( Yu et al., 2005 ).”
Human gut microbiota in obesity and after gastric bypass (2009, methodology): “To quantify Bacteria, Archaea, and the archaeal subgroups, we performed 16S rRNA gene-targeted QPCR with either SYBR-green (for Bacteria) or TaqMan detection (for Archaea, Methanobacteriales, Methanomicrobiales, and Methanosaetaceae) (37, 38).”
Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR (2007, methodology): “An exception was the domain-level eubacterial pair, which was designed by Yu et al. (2005) to amplify a total of 468 bp and to have lower Tm values (see Table 2).”
Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing (2014, background): “We also demonstrated that the universal prokaryotic primer set designed here has markedly lower bias than that of most previously applied universal primers [23,40].”
Constraints on the Biological Source(s) of the Orphan Branched Tetraether Membrane Lipids (2009, methodology): “Of the primer pairs specific to the three most important orders of methanogenic archaea, i.e., Methanomicrobiales, Methanobacteriales and Methanosarcinales, only the primer pair for Methanomicrobiales gave positive results, indicating that Methanobacteriales and Methanosarcinales are either not pres”