CytoBuoy: a step forward towards using flow cytometry in operational oceanography*
By G. Dubelaar, P. L. Gerritzen. 2000
122 citations recorded by the source corpus.
SUMMARY: While the performance of biological sensors in real time monitoring networks is limited to bulk values like chlorophyll fluorescence, in practice the implementation of automated phytoplankton taxonomy remains a remote option. Aiming to reduce this gap we developed a flow cytometer called CytoBuoy for autonomous in situ operation, for instance in a moored buoy with wireless data transfer. Although not comparable to microscopy, flow cytometers detect and count particles allowing a limited level of particle characterization based on the light scatter and fluorescence properties of the individual particles. CytoBuoy analyses a large size range of particles, typical for marine coastal zones and fresh waters. The ‘field’ design implies a tradeoff between the accuracy and versatility of laboratory flow cytometers and the qualities needed for trouble free autonomous operation in situ. The optics and electronics however were designed for maximal reflection of the particle morphology in the measured signals. Whereas standard cytometers reduce these to single peak or area ‘listmode’ numbers, the signal courses are preserved fully by CytoBuoy and transferred to the computer as raw data, which allows more extended morphological analysis. Extended field tests will have to show how the system holds in various environments and weather conditions.
Subfields: Oceanography
View Paper: https://www.semanticscholar.org/paper/de07f535019b6f03c942754d6519549c09b848d6
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In Oceanography
Citations in the corpus
- LISST‐100 measurements of phytoplankton size distribution: evaluation of the effects of cell shape (2007, unknown): “5 mL/s) compared to the volume sampled by an instrument that analyzes individual particles (e.g., <0.005 μL/s for the CytoBuoy; Dubelaar and Gerritzen, 2000).”
- The effect of nutrient availability and temperature on chain length of the diatom, Skeletonema costatum (2006, methodology): “With the aid of a recently developed flow cytometer, CytoSense and its analytical software packages, CytoSift and CytoClus (CytoBuoy b.v., Nieuwerbrug, Netherlands; Dubelaar and Gerritzen, 2000), which makes enumeration of diatom chains and individual cells less laborious than the traditional visual”
- Application of flow cytometry in marine phytoplankton research: current applications and future perspectives (2000, methodology): “…features more sophisticated instruments have been designed like the Optical Plankton Analyzer (OPA), its successor EUROPA (Peeters et al., 1989; Dubelaar et al., 1989; Peperzak et al., 2000) and more recently its small scale version CytoBuoy (Dubelaar et al., 1999, Dubelaar and Gerritzen, 2000).”
- Selective grazing by adults and larvae of the zebra mussel (Dreissena polymorpha): application of flow cytometry to natural seston (2004, unknown): “With improvement in discriminating be-tween diatoms and green algae and the development of in situ flow cytometers (Dubelaar et al. , 1999; Dubelaar & Gerritzen, 2000), flow cytometry has the potential to become the preferred tool for measuring grazing on heterogeneous assemblages of particles.”
- Flow cytometry as a tool for the study of phytoplankton (2000, unknown): “1) to shore (Dubelaar et al., 1999; Dubelaar and Gerritzen, 2000).”