Flow cytometry as a tool for the study of phytoplankton

By G. Dubelaar, R. Jonker. 2000

124 citations recorded by the source corpus.

An overview is presented on flow cytometry as a tool for counting, analysis and identification of phytoplankton species and groups. The paper covers basics on the analysis technique and instrumentation such as the measuring principle, the type of instrument, limitations and pitfalls with phytoplankton samples and sample handling and preprocessing. Possibilities of the measured entities are discussed, roughly divided in light scatter and related parameters, the endogenous fluorescence and exogenous fluorescence, followed by a discussion on the actual applications such as phytoplankton abundance analysis, ecology and physiology research and monitoring of particle size and biomass. In addition to a limited literature review, we tried to assess how flow cytometry is used in routine laboratory practice and monitoring operations. Therefore, a questionnaire was sent out via email to 47 scientists at 43 institutes known to us as involved in flow cytometric analysis of phytoplankton. In total, 19 scientists responded. Specific survey results are included in italic print whereas some more general answers were integrated in the overview.

Subfields: Ecology, Oceanography, Pollution

View Paper: https://www.semanticscholar.org/paper/b83bc27b69e31071cc7e52207a8672ed1b88c425

Citations in the corpus

• DON as a source of bioavailable nitrogen for phytoplankton (2006, unknown): “…et al., 1983; Chisholm et al., 1988), flow cytometry now has a wide variety of applications in aquatic microbial ecology (e.g. Wallner et al., 1997; Dubelaar and Jonker, 2000; Reckermann, 2000; Veldhuis and Kraay, 2000; Vives-Rego et al., 2000; Campbell, 2001; Collier, 2004), including routine…”
• Methods for sampling free-living Symbiodinium (zooxanthellae) and their distribution and abundance at Lizard Island (Great Barrier Reef) (2008, unknown): “(Fitt, 1984; Pasternak et al., 2006; Yacobovitch et al., 2004). Furthermore, disparities in host abundance, as a source of zooxanthellae may contribute to the differences in freeliving Symbiodium among and within reef sites. Jones and Yellowlees (1997) proposed that corals can continuously release z”
• Selective grazing by adults and larvae of the zebra mussel (Dreissena polymorpha): application of flow cytometry to natural seston (2004, unknown): “The application of flow cytometry in grazing studies is increasing (Dubelaar & Jonker, 2000), especially in marine waters (Cucci et al. , 1985, 1989; Shumway et al. , 1985; Bougrier, Hawkins & He´ral, 1997; Recker-mann & Veldhuis, 1997; Ward et al. , 1997, 1998; Christaki et al. , 1999; Kuipers &…”