Application of flow cytometry in marine phytoplankton research: current applications and future perspectives

By M. Veldhuis, G. Kraay. 2000

206 citations recorded by the source corpus.

A brief overview is given of current applications of flow cytometry (FCM) in marine phytoplankton research. This paper presents a selection of highlights and various technical and analytical problems we encountered during the past 10 years. In particular, the conversion of the relative values obtained in terms of size and fluorescence applying FCM to quantitative estimates of cell size, pigment concentration, genome size etc., is addressed. The introduction of DNA -cell-cycle analysis made easily assessable by flow cytometry has been of great importance, allowing in situ measurement of species specific growth rates. Key questions in ecology such as factors determining the wax and wane of phytoplankton bloom can now be better answered in terms of species specific growth and mortality. Finally, flow cytometry provides detailed information of the physiological status of the individual algal cells. New staining methods enable us to distinguish between viable and non-viable cells and so will help us to elucidate the importance of automortality in aquatic ecosystems.

Subfields: Ecology, Global and Planetary Change, Oceanography

View Paper: https://www.semanticscholar.org/paper/16c7c4731efc7ecc51f5605a8496352e6f2f659e

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. Filip Meysman
2. E. Zetsche
3. C. Brunet
4. A. Ianora
5. A. Miralto
6. M. Veldhuis
7. F. Jochem
8. S. Mazza

In Global and Planetary Change

1. F. Jochem
2. Filip Meysman
3. E. Zetsche
4. M. Veldhuis

In Oceanography

1. M. Veldhuis
2. F. Jochem
3. Ilka Peeken
4. C. Brunet
5. P. Assmy
6. K. Lochte
7. L. Hoffmann
8. Filip Meysman

Citations in the corpus

• DON as a source of bioavailable nitrogen for phytoplankton (2006, unknown): “…now has a wide variety of applications in aquatic microbial ecology (e.g. Wallner et al., 1997; Dubelaar and Jonker, 2000; Reckermann, 2000; Veldhuis and Kraay, 2000; Vives-Rego et al., 2000; Campbell, 2001; Collier, 2004), including routine analysis of the abundance of marine…”
• Different reactions of Southern Ocean phytoplankton size classes to iron fertilization (2006, methodology): “Flow cytometer samples were analyzed with a bench-top flow cytometer (Beckman Coulter XL-MCL) as described by Veldhuis and coworkers (Veldhuis and Kraay 2000).”
• Dead or alive? Viability assessment of micro- and mesoplankton (2012, background): “…ic.oup.com /plankt/article-abstract/34/6/493/1573861 by guest on 01 January 2019 suggest a strong impact of cell death on marine ecosystem functioning, particularly on our understanding of dissolved organic carbon cycling (Veldhuis and Kraay, 2000; Agustı́ et al., 2006; Hayakawa et al., 2008).”
• Picophytoplankton - a comparative study of their biochemical composition and photosynthetic properties (2005, unknown): “2 d 1 (Veldhuis and Kraay, 2000).”
• Probing the physiological state of phytoplankton at the single-cell level (2000, background): “New insights into community dynamics such as taxonomy, specific activity or limiting factors are nowadays possible through the development of new optical and molecular probes of individual cell physiology: quantification of cell death (Veldhuis and Kraay, 2000), reproduction (Jochem and Meyerdierks,”
• GROWTH INHIBITION AND TOXICITY OF THE DIATOM ALDEHYDE 2‐TRANS, 4‐TRANS‐DECADIENAL ON THALASSIOSIRA WEISSFLOGII (BACILLARIOPHYCEAE) 1 (2005, methodology): “For this reason, it is used to determine cell viability (Roth et al. 1997) and to estimate membrane integrity in phytoplankton and bacteria (Lebaron et al. 1998, Veldhuis and Kraay 2000).”
• CytoBuoy: a step forward towards using flow cytometry in operational oceanography* (2000, background): “Particle size and measurement principle The oceans are dominated by small single phytoplankton cells like Synechococcus sp. and the Prochlorophytes (Veldhuis and Kraay, 2000).”