COMPARATIVE STUDIES ON PROTEOLYTIC ACTIVITY OF SPLENIC EXTRACT FROM THREE TUNA SPECIES COMMONLY USED IN THAILAND

By S. Klomklao, Sootawat Benjakul, Wonnop Visessanguan. 2004

132 citations recorded by the source corpus.

Proteolytic activities of splenic extract from three tuna species including skipjack tuna (Katsuwonus pelamis) , yellowfin tuna (Thunnus albacores) and tongol tuna (Thunnus tonggol) were studied. Optimal activity of splenic extract from all tuna species was at pH 9.0 and 55C when casein was used as a substrate. Among all species tested, yellowfin tuna showed the highest activity, followed by skipjack tuna and tongol tuna. The proteolytic activity was strongly inhibited by soybean trypsin inhibitor, TLCK and partially inhibited by ethylenediaminetetraacetic acid. E-64, N-ethylmaleimide, iodoacetic acid, TPCK and pepstatin A showed no inhibition. The effect of NaCl and CaCl 2 on proteolytic activity was also investigated. Activities continuously decreased as NaCl concentration increased, and no activity remained in the presence of 30% NaCl. On the other hand, activities increased as CaCl 2 concentration increased. The highest activity was obtained in the presence of 1 mM CaCl 2 . SDS-substrate gel electrophoresis revealed that major proteinases in splenic extract from different tuna species were different in apparent molecular weights and sensitivity to TLCK. Although the major activity bands

Subfields: Aquatic Science, Biotechnology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/daa2583849782e89279225ae12834f4754e4e740

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Aquatic Science

1. S. Benjakul
2. S. Budge
3. A. Ghaly
4. S. Iverson

In Biotechnology

1. D. Dave
2. A. Ghaly

In Molecular Biology

1. H. Kishimura
2. S. Benjakul
3. Wonnop Visessanguan
4. M. Brooks
5. S. Budge
6. D. Dave
7. A. Ghaly
8. P. Szpak

Citations in the corpus

• Partitioning and recovery of proteinase from tuna spleen by aqueous two-phase systems (2005, background): “In terms of volume, Thailand is the world's largest exporter of canned tuna, for over 20 million cans annually during the past 5 years [1,2].”
• Fish Spoilage Mechanisms and Preservation Techniques: Review (2010, background): “Sen (2005) reported that 10% solution of sodium chloride inactivated the autolytic enzyme when deveind shrimp was blanched for 5 minutes at 80°C. Klomklao et al. (2004) studied proteolytic activities of splenic extract from three tuna species: skipjack tuna (Katsuwonus pelamis), yellowfin tuna…”