Elongation by RNA polymerase II: the short and long of it.

By R. Sims, R. Belotserkovskaya, D. Reinberg. Genes & Development, 2004

770 citations recorded by the source corpus.

Appreciable advances into the process of transcript elongation by RNA polymerase II (RNAP II) have identified this stage as a dynamic and highly regulated step of the transcription cycle. Here, we discuss the many factors that regulate the elongation stage of transcription. Our discussion includes the classical elongation factors that modulate the activity of RNAP II, and the more recently identified factors that facilitate elongation on chromatin templates. Additionally, we discuss the factors that associate with RNAP II, but do not modulate its catalytic activity. Elongation is highlighted as a central process that coordinates multiple stages in mRNA biogenesis and maturation.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d87958e4b7e45def58ae14311f3b0b9dc90ad9d5

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Citations in the corpus

Intermingling of Chromosome Territories in Interphase Suggests Role in Translocations and Transcription-Dependent Associations (2006, background): “phosphorylated (active [37]) form of PolII are present within”
H3K4me3 breadth is linked to cell identity and transcriptional consistency (2014, background): “, super elongation complex, P-TEFb) (p < 1 3 10 4 in permutation test; Figures 5A, 5B, and S5A–S5E) (Luo et al., 2012; Sims et al., 2004).”
Genomic maps and comparative analysis of histone modifications in human and mouse. (2005, methodology): “Elongation promotes chroDNA Amplification and Array Hybridizationmatin opening (Sims et al., 2004), and nongenic tranChIP (enriched) and control (whole-cell extract) DNA samples werescripts appear to enhance the accessibility of the amplified by in vitro transcription as described (Liu et al.,…”
A chromatin landmark and transcription initiation at most promoters in human cells. (2007, background): “This may be due to transcriptional pausing, poor processivity, or abortive initiation, all of which have been described as mechanisms that prevent initiating polymerase from elon-gating efﬁciently through speciﬁc genes (Conaway et al., 2000; Dvir, 2002; Sims et al., 2004; Saunders et al., 2006).”
Targeting MYC dependence in cancer by inhibiting BET bromodomains (2011, neutral): “SKNDZ NCI-H69 BE(2)C CHP212 SNU182 - + - + - + - +”
Recognition of trimethylated histone H3 lysine 4 facilitates the recruitment of transcription postinitiation factors and pre-mRNA splicing. (2007, background): “Transcript elongation is a highly regulated and dynamic stage of the transcription cycle (Sims et al., 2004).”
Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II. (2006, background): “PAF Complex and H2BK120ub1 Cooperatively Stimulate FACT-Dependent Transcription We next tested whether the PAF complex and H2BK120ub1 might stimulate FACT-dependent transcription based on their reported interaction (Sims et al., 2004).”
Phosphorylation and functions of the RNA polymerase II CTD. (2006, background): “This overview contains many features of recently published models (Sims et al. 2004; Zorio and Bentley 2004), but it differs from the others in explicitly including proteins that bind to Ser2,5P repeats as major components of the elongating complex.”