Circulating tumor cells: approaches to isolation and characterization

By Min Yu, Shannon L. Stott, Mehmet Toner, S. Maheswaran, D. Haber. Journal of Cell Biology, 2011

1,085 citations recorded by the source corpus.

Circulating tumor cells (CTCs) shed from primary and metastatic cancers are admixed with blood components and are thus rare, making their isolation and characterization a major technological challenge. CTCs hold the key to understanding the biology of metastasis and provide a biomarker to noninvasively measure the evolution of tumor genotypes during treatment and disease progression. Improvements in technologies to yield purer CTC populations amenable to better cellular and molecular characterization will enable a broad range of clinical applications, including early detection of disease and the discovery of biomarkers to predict treatment responses and disease progression.

Subfields: Cancer Research, Oncology

View Paper: https://www.semanticscholar.org/paper/d3f1e3971161d1f55cc5bf88ff6bd9f041002891

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Citations in the corpus

Technologies for Single-Cell Isolation (2015, background): “generation sequencing (NGS) of single cells [82], isolation of circulating tumor cells for diagnostic purposes [83], or single-cell proteomics [84].”
Epithelial–mesenchymal plasticity in carcinoma metastasis (2013, methodology): “This may be due in part to the challenges in capturing CTCs that have fully undergone EMT, given that current isolation methods rely primarily on using epithelial markers, such as EpCam, to capture CTCs from the blood or bone marrow (Yu et al. 2011).”
Circulating Tumor Cell Clusters are Oligoclonal Precursors of Breast Cancer Metastasis (2014, methodology): “CTCs have been detected in the majority of epithelial cancers, where they represent cancer cells captured as they transit through the bloodstream (Alix-Panabières and Pantel, 2013; Yu et al., 2011).”